Detailed information of TA system TA030313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 3145..3704
• Replicon: plasmid pHUSEC2011-2
• Accession: NC_022743
• Organism: Escherichia coli

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G4VUF8
• Locus tag: HUS2011_RS27990
• Protein ID: WP_000421273.1
• Coordinates: 3145..3420 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: HUS2011_RS27995
• Protein ID: WP_001387467.1
• Coordinates: 3420..3704 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS2011_RS27965	166..1143	+	978	WP_001387465.1	RepB family plasmid replication initiator protein	-
HUS2011_RS27970	1422..2162	-	741	WP_001066951.1	tyrosine-type recombinase/integrase	-
HUS2011_RS27975	2283..2408	-	126	WP_032159539.1	hypothetical protein	-
HUS2011_RS27980	2531..2692	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HUS2011_RS28665	2808..3139	-	332	Protein_4	type II toxin-antitoxin system ParD family antitoxin	-
HUS2011_RS27990	3145..3420	-	276	WP_000421273.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUS2011_RS27995	3420..3704	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
HUS2011_RS28000	4612..5440	-	829	Protein_7	incFII family plasmid replication initiator RepA	-
HUS2011_RS28010	5433..5507	-	75	WP_014966221.1	RepA leader peptide Tap	-
HUS2011_RS32060	5504..5638	-	135	Protein_9	protein CopA/IncA	-
HUS2011_RS28015	5745..5999	-	255	WP_000083850.1	replication regulatory protein RepA	-
HUS2011_RS28020	6280..7260	+	981	WP_000019440.1	IS5-like element ISKpn26 family transposase	-
HUS2011_RS28025	7711..7923	-	213	Protein_12	ANR family transcriptional regulator	-
HUS2011_RS28030	7988..8665	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	aap/aspU / aggA / aggB / aggC / aggD	1..75569	75569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10765.45 Da
• Isoelectric Point: 9.9324

>T30313 WP_000421273.1 NC_022743:c3420-3145 [Escherichia coli]
MELKWTSKALSDLSRLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T30313 NC_022743:c3420-3145 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT30313 WP_001387467.1 NC_022743:c3704-3420 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT30313 NC_022743:c3704-3420 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y6H1

Antitoxin

• AlphaFold DB: A0A0E0Y880