Detailed information of TA system TA030292

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 5415311..5415986
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZNR6
• Locus tag: MKAN_RS23390
• Protein ID: WP_023372362.1
• Coordinates: 5415311..5415742 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X7ZS36
• Locus tag: MKAN_RS23395
• Protein ID: WP_036391998.1
• Coordinates: 5415729..5415986 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS23360	5410683..5411240	+	558	WP_023372356.1	ribosome recycling factor	-
MKAN_RS23365	5411272..5412168	+	897	WP_023372357.1	phosphatidate cytidylyltransferase	-
MKAN_RS23370	5412210..5413304	+	1095	WP_023372358.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
MKAN_RS23375	5413545..5414066	+	522	WP_023372359.1	protein disulfide oxidoreductase	-
MKAN_RS23380	5414071..5414937	+	867	WP_023372360.1	cytochrome c biogenesis CcdA family protein	-
MKAN_RS23385	5414930..5415184	-	255	WP_023372361.1	DUF2631 domain-containing protein	-
MKAN_RS23390	5415311..5415742	-	432	WP_023372362.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKAN_RS23395	5415729..5415986	-	258	WP_036391998.1	ribbon-helix-helix domain-containing protein	Antitoxin
MKAN_RS23400	5416074..5417279	+	1206	WP_036392002.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
MKAN_RS23405	5417299..5418513	+	1215	WP_023372365.1	M50 family metallopeptidase	-
MKAN_RS23410	5418537..5419712	+	1176	WP_023372366.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
MKAN_RS23415	5419765..5420622	+	858	WP_023372367.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 16024.11 Da
• Isoelectric Point: 6.3292

>T30292 WP_023372362.1 NC_022663:c5415742-5415311 [Mycobacterium kansasii ATCC 12478]
MHCVDVNVLVNAHRADLREHPNYRDVLERLANGDEPLGLPDSVLAGFVRVVTNRRIFVEPTSQPDAWQAVDALLAAPAAI
RLKPGERHWTLFRQLAADIDASGNDIADAYLAAYALENNATWVSADRGFARFGRLRWRHPLDM

Nucleotide

• Length: 432 bp

>T30292 NC_022663:c5415742-5415311 [Mycobacterium kansasii ATCC 12478]
ATGCATTGCGTTGACGTCAACGTTCTCGTCAACGCCCATCGAGCCGACCTGCGAGAACACCCGAACTACCGAGATGTACT
CGAGCGGCTGGCCAACGGCGACGAGCCGCTCGGTCTGCCGGACAGCGTGCTCGCCGGCTTCGTCCGGGTGGTGACCAACC
GCCGCATCTTCGTCGAACCGACCAGCCAGCCCGACGCATGGCAGGCGGTGGATGCCTTGCTCGCGGCACCCGCCGCGATA
CGGCTCAAGCCCGGCGAGCGGCACTGGACGCTGTTTCGGCAGCTCGCCGCCGATATCGATGCGAGTGGCAACGACATCGC
GGACGCGTATCTGGCCGCCTACGCCCTGGAGAACAACGCGACCTGGGTGAGCGCAGACCGCGGTTTCGCCCGCTTTGGCC
GGCTGCGCTGGCGTCATCCGCTGGATATGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 8917.06 Da
• Isoelectric Point: 7.9812

>AT30292 WP_036391998.1 NC_022663:c5415986-5415729 [Mycobacterium kansasii ATCC 12478]
VRTTIRIDDELYREVKARAARSGRTVAAVLEDAVRCGLNPPGQSAAGRYRVQPIGSGGLRPGVDLSSNAAVAEAMDEGVP
VDALR

Nucleotide

• Length: 258 bp

>AT30292 NC_022663:c5415986-5415729 [Mycobacterium kansasii ATCC 12478]
GTGCGCACGACGATCCGGATCGACGACGAGCTCTACCGCGAGGTGAAGGCACGGGCCGCTCGATCCGGGCGTACCGTGGC
CGCGGTTCTCGAAGATGCGGTGCGATGCGGTCTCAATCCGCCCGGGCAGTCGGCGGCCGGGCGTTATCGAGTCCAGCCGA
TTGGCAGCGGCGGGCTTAGACCCGGCGTGGATCTGTCGTCGAACGCCGCGGTCGCAGAGGCGATGGACGAGGGCGTGCCG
GTCGATGCATTGCGTTGA

Structures

Toxin

• AlphaFold DB: X7ZNR6

Antitoxin

• AlphaFold DB: X7ZS36