Detailed information of TA system TA030289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3952886..3953574
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7Z9W3
• Locus tag: MKAN_RS17195
• Protein ID: WP_023370286.1
• Coordinates: 3952886..3953317 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MKAN_RS17200
• Protein ID: WP_036394775.1
• Coordinates: 3953314..3953574 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS17160	3948416..3948724	+	309	WP_023370272.1	DUF3263 domain-containing protein	-
MKAN_RS17165	3948789..3949259	+	471	WP_036394685.1	LytR C-terminal domain-containing protein	-
MKAN_RS17170	3949378..3950094	+	717	WP_023370276.1	superoxide dismutase family protein	-
MKAN_RS17175	3950075..3951238	+	1164	WP_103797770.1	glutamate--cysteine ligase	-
MKAN_RS30595	3951321..3951722	+	402	WP_103797769.1	hypothetical protein	-
MKAN_RS30600	3951794..3952033	+	240	WP_155254706.1	hypothetical protein	-
MKAN_RS17190	3952230..3952877	+	648	WP_023370284.1	LON peptidase substrate-binding domain-containing protein	-
MKAN_RS17195	3952886..3953317	-	432	WP_023370286.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKAN_RS17200	3953314..3953574	-	261	WP_036394775.1	ribbon-helix-helix domain-containing protein	Antitoxin
MKAN_RS17205	3953619..3955823	-	2205	WP_023370290.1	AAA family ATPase	-
MKAN_RS17210	3955820..3956680	-	861	WP_023370292.1	CDP-diacylglycerol--serine O-phosphatidyltransferase	-
MKAN_RS17215	3956677..3957372	-	696	WP_023370294.1	phosphatidylserine decarboxylase	-
MKAN_RS29790	3957493..3957724	-	232	Protein_3443	molybdopterin molybdenumtransferase MoeA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-134)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-40)

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15954.13 Da
• Isoelectric Point: 5.0701

>T30289 WP_023370286.1 NC_022663:c3953317-3952886 [Mycobacterium kansasii ATCC 12478]
VIIPDVNLLLYAVITGFPQHRRAYAWWHDTVNSQERIGLTYPAVFGFLRIVTNARILTEPLSATEAVAYVRDWLTQPNVD
LLIPSARHLDLALGLLEQLGTASNLTTDVQLAAYAIEHNAEMHSSDADFARFAGLAWTNPLDR

Nucleotide

• Length: 432 bp

>T30289 NC_022663:c3953317-3952886 [Mycobacterium kansasii ATCC 12478]
GTGATCATCCCCGACGTCAATCTGCTGCTGTACGCGGTCATCACGGGCTTCCCACAGCATCGGCGCGCGTACGCCTGGTG
GCACGACACCGTCAACAGCCAAGAGCGCATCGGGCTGACATACCCGGCCGTTTTCGGCTTTTTGCGTATCGTGACCAACG
CACGCATCCTCACCGAGCCCCTGTCTGCGACCGAAGCAGTCGCATATGTGCGTGACTGGCTTACGCAGCCGAACGTGGAC
CTTCTTATCCCGTCTGCCCGCCATCTGGATCTGGCATTGGGGTTGCTTGAGCAGCTCGGCACGGCCAGCAACCTGACCAC
CGACGTCCAACTGGCCGCCTACGCGATCGAGCACAACGCCGAGATGCATTCCAGCGACGCTGATTTCGCCCGATTCGCCG
GTCTCGCGTGGACCAACCCACTTGACCGATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10093.61 Da
• Isoelectric Point: 9.9864

>AT30289 WP_036394775.1 NC_022663:c3953574-3953314 [Mycobacterium kansasii ATCC 12478]
MRTTLTLDDDVVRLIEEAVHRERRPMKHVINDALRKALAQPMERRTPYQLKPHRSAVRPGFDFAGFNRLADEMEDQAILD
QKRPTL

Nucleotide

• Length: 261 bp

>AT30289 NC_022663:c3953574-3953314 [Mycobacterium kansasii ATCC 12478]
ATGCGCACCACTTTGACGCTCGACGACGACGTCGTTCGCCTGATCGAAGAGGCTGTTCATCGCGAACGCCGCCCCATGAA
GCACGTCATCAACGACGCGCTGCGCAAAGCGTTGGCGCAGCCGATGGAACGCCGGACGCCGTATCAGTTGAAGCCGCATC
GCTCGGCAGTGCGGCCGGGATTCGATTTTGCCGGGTTCAACCGGCTGGCCGACGAGATGGAAGACCAAGCGATCCTGGAC
CAGAAGCGACCGACCCTGTGA

Structures

Toxin

• AlphaFold DB: X7Z9W3