Detailed information of TA system TA030288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2095274..2095895
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MKAN_RS08895
• Protein ID: WP_023367403.1
• Coordinates: 2095274..2095711 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X7ZGA3
• Locus tag: MKAN_RS08900
• Protein ID: WP_023367405.1
• Coordinates: 2095716..2095895 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS08870	2091067..2092581	+	1515	WP_036393600.1	carotenoid oxygenase family protein	-
MKAN_RS08875	2092661..2093143	+	483	WP_023367395.1	VOC family protein	-
MKAN_RS08880	2093149..2093886	-	738	WP_023367397.1	trimeric intracellular cation channel family protein	-
MKAN_RS08885	2093928..2094347	-	420	WP_023367399.1	hypothetical protein	-
MKAN_RS08890	2094384..2095166	-	783	WP_036393899.1	VOC family protein	-
MKAN_RS08895	2095274..2095711	-	438	WP_023367403.1	SRPBCC family protein	Toxin
MKAN_RS08900	2095716..2095895	-	180	WP_023367405.1	antitoxin	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-141)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15888.48 Da
• Isoelectric Point: 8.5679

>T30288 WP_023367403.1 NC_022663:c2095711-2095274 [Mycobacterium kansasii ATCC 12478]
MAKLSGSIDVPLPPEEAWLHASNLSRYREWLTIHRVWRSKLPETLERGAVVESIVEVKGMPNRIRWTIVRYKPPEGMTLN
GDGVGGVKVKLMAKVAPKEEGSVVSFDVHLGGPALFGPIGMIVAAALRSDISESLQNFVEVFAQA

Nucleotide

• Length: 438 bp

>T30288 NC_022663:c2095711-2095274 [Mycobacterium kansasii ATCC 12478]
ATGGCGAAACTCTCCGGATCCATCGATGTCCCGTTGCCGCCGGAGGAGGCGTGGCTGCACGCCTCGAATCTGTCCCGCTA
CCGGGAGTGGCTGACGATCCACCGGGTCTGGCGCAGCAAGTTGCCCGAAACGCTGGAGAGGGGCGCGGTCGTCGAGTCGA
TCGTCGAGGTCAAGGGGATGCCCAACCGGATCAGATGGACGATCGTGCGCTACAAGCCGCCGGAAGGCATGACGCTCAAC
GGCGACGGAGTCGGCGGCGTCAAAGTCAAACTCATGGCCAAGGTCGCGCCCAAAGAGGAGGGCTCCGTCGTGAGCTTCGA
CGTGCACCTCGGCGGACCCGCCCTGTTCGGGCCGATCGGCATGATCGTCGCCGCCGCGCTCAGAAGCGACATCAGCGAAT
CCCTGCAGAACTTCGTCGAGGTGTTCGCACAGGCCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6552.35 Da
• Isoelectric Point: 5.7603

>AT30288 WP_023367405.1 NC_022663:c2095895-2095716 [Mycobacterium kansasii ATCC 12478]
MGIMDKAKDLLSQNADKVETVIDKAGKFVDDKTQGKYSDTIHKVQEQAKKVVDTGDQQN

Nucleotide

• Length: 180 bp

>AT30288 NC_022663:c2095895-2095716 [Mycobacterium kansasii ATCC 12478]
ATGGGAATCATGGACAAGGCAAAAGACCTGCTGTCACAGAACGCCGACAAGGTCGAGACGGTGATCGACAAGGCCGGCAA
ATTCGTCGACGACAAGACGCAGGGCAAGTACTCCGACACCATTCACAAGGTGCAGGAGCAGGCCAAGAAAGTAGTCGACA
CGGGCGACCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: X7ZGA3