Detailed information of TA system TA030286

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1228551..1229243
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZNI0
• Locus tag: MKAN_RS05245
• Protein ID: WP_023365904.1
• Coordinates: 1228821..1229243 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: X7ZK45
• Locus tag: MKAN_RS05240
• Protein ID: WP_036392951.1
• Coordinates: 1228551..1228814 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS05210	1223623..1225125	-	1503	WP_023365894.1	trigger factor	-
MKAN_RS05225	1225486..1226670	+	1185	WP_023365896.1	serine hydrolase	-
MKAN_RS30300	1226717..1227043	-	327	WP_133163539.1	hypothetical protein	-
MKAN_RS05230	1227131..1227937	-	807	WP_023365898.1	Fpg/Nei family DNA glycosylase	-
MKAN_RS05235	1227971..1228459	-	489	WP_099184200.1	ribose-5-phosphate isomerase	-
MKAN_RS05240	1228551..1228814	+	264	WP_036392951.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MKAN_RS05245	1228821..1229243	+	423	WP_023365904.1	type II toxin-antitoxin system VapC family toxin	Toxin
MKAN_RS05250	1229240..1229851	-	612	WP_023365906.1	Rv2466c family mycothiol-dependent reductase	-
MKAN_RS05255	1229992..1232595	+	2604	WP_036392948.1	aminopeptidase N	-
MKAN_RS05260	1232663..1233130	-	468	WP_036392579.1	DUF5130 domain-containing protein	-
MKAN_RS05265	1233117..1233344	-	228	WP_023365912.1	hypothetical protein	-
MKAN_RS05270	1233407..1234081	-	675	WP_036392577.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-83)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15092.17 Da
• Isoelectric Point: 5.0719

>T30286 WP_023365904.1 NC_022663:1228821-1229243 [Mycobacterium kansasii ATCC 12478]
VILDSSAFVALIQGEAPYTEQIAAALTGDRSPVMSTANAAECLIVLTSRHGFIARTVFDRLRFEINLGFQPFNFELAMAA
HLAYLRYGRGRHPAGLNCGDTMAYATAKLAHEPLIAIGNDFAQTDLESDGVIGYWPSPCG

Nucleotide

• Length: 423 bp

>T30286 NC_022663:1228821-1229243 [Mycobacterium kansasii ATCC 12478]
GTGATCCTCGACTCGTCGGCCTTCGTCGCGCTCATCCAGGGCGAGGCTCCCTACACCGAACAGATCGCGGCAGCTCTCAC
TGGGGACCGAAGTCCGGTCATGTCAACGGCGAACGCAGCTGAATGCCTGATCGTGCTCACTAGCCGACACGGCTTCATCG
CCCGCACCGTCTTCGACCGCCTGCGATTCGAAATCAACCTCGGCTTCCAGCCGTTCAACTTCGAGCTCGCGATGGCCGCG
CATCTCGCATACCTGCGATATGGAAGGGGCCGACACCCTGCTGGCCTCAACTGCGGGGACACCATGGCCTACGCCACCGC
CAAACTCGCGCACGAACCGCTCATCGCGATCGGCAATGACTTCGCTCAAACAGACCTCGAATCCGACGGTGTCATCGGCT
ACTGGCCATCGCCCTGCGGCTAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9890.16 Da
• Isoelectric Point: 5.6277

>AT30286 WP_036392951.1 NC_022663:1228551-1228814 [Mycobacterium kansasii ATCC 12478]
VAFNVKDEEVIRLADELAARLHHPSRIDAIRYALRAQIEITQSRTANRADELLDVLRTEIWPLLHDRSPITKSEREQALG
YDAATGV

Nucleotide

• Length: 264 bp

>AT30286 NC_022663:1228551-1228814 [Mycobacterium kansasii ATCC 12478]
GTGGCATTCAACGTCAAGGATGAAGAGGTGATCCGCTTGGCCGATGAGCTCGCCGCACGTCTTCACCACCCGAGCCGCAT
CGACGCTATCCGGTATGCACTGCGCGCCCAGATCGAGATCACGCAGTCCCGCACTGCAAACCGTGCCGATGAACTCCTCG
ACGTCCTCAGAACCGAGATCTGGCCATTGCTGCACGATCGATCCCCGATCACCAAGTCCGAACGCGAACAAGCCCTCGGA
TACGACGCGGCGACGGGCGTGTGA

Structures

Toxin

• AlphaFold DB: X7ZNI0

Antitoxin

• AlphaFold DB: X7ZK45