Detailed information of TA system TA030285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 1085130..1085707
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: doc
• Uniprot ID: X7ZN33
• Locus tag: MKAN_RS04645
• Protein ID: WP_023365653.1
• Coordinates: 1085324..1085707 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: X7ZMP8
• Locus tag: MKAN_RS04640
• Protein ID: WP_023365651.1
• Coordinates: 1085130..1085327 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS04615	1080274..1080810	-	537	WP_023365641.1	rRNA maturation RNase YbeY	-
MKAN_RS04620	1080860..1081909	-	1050	WP_036392639.1	PhoH family protein	-
MKAN_RS04625	1082056..1083501	-	1446	WP_133163534.1	cytochrome P450	-
MKAN_RS04630	1083584..1084375	-	792	WP_023365647.1	class I SAM-dependent methyltransferase	-
MKAN_RS04635	1084445..1085014	-	570	WP_023365649.1	carboxymethylenebutenolidase	-
MKAN_RS04640	1085130..1085327	+	198	WP_023365651.1	hypothetical protein	Antitoxin
MKAN_RS04645	1085324..1085707	+	384	WP_023365653.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MKAN_RS04650	1085730..1086476	-	747	WP_023365655.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
MKAN_RS04655	1086495..1087643	-	1149	WP_023365657.1	molecular chaperone DnaJ	-
MKAN_RS04660	1087703..1088734	-	1032	WP_023365659.1	heat-inducible transcriptional repressor HrcA	-
MKAN_RS04665	1088950..1089264	+	315	WP_023365661.1	type II toxin-antitoxin system VapB family antitoxin	-
MKAN_RS04670	1089422..1089771	+	350	Protein_945	hypothetical protein	-
MKAN_RS04675	1089815..1090382	+	568	Protein_946	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (19-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13726.39 Da
• Isoelectric Point: 3.9949

>T30285 WP_023365653.1 NC_022663:1085324-1085707 [Mycobacterium kansasii ATCC 12478]
VTEFFDRDDVLTAGALAVGAVLEVGDFGLLDAAVARPQATVYGVDAYPDLYTKAAALLQSLARNRALVDGNKRTAWASAW
TFLYINGIELVAGFDVDTAERFMYDVAERGELTVDDIAETLRQFASA

Nucleotide

• Length: 384 bp

>T30285 NC_022663:1085324-1085707 [Mycobacterium kansasii ATCC 12478]
GTGACGGAATTCTTCGATCGTGACGACGTCCTCACCGCCGGCGCGCTCGCCGTGGGCGCGGTGCTCGAGGTCGGCGACTT
CGGGCTGCTCGACGCCGCGGTCGCGCGACCTCAGGCCACCGTTTACGGGGTCGACGCCTACCCCGATCTCTATACCAAGG
CAGCGGCGCTGTTACAGTCGCTGGCCCGCAACCGTGCTCTGGTAGACGGCAATAAGCGAACCGCCTGGGCGTCGGCGTGG
ACGTTCTTGTATATCAACGGGATCGAGCTTGTGGCCGGGTTTGATGTAGACACCGCCGAGAGGTTCATGTACGACGTAGC
CGAGCGCGGTGAACTGACCGTCGACGACATCGCCGAAACACTGCGCCAGTTCGCGTCCGCCTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6940.89 Da
• Isoelectric Point: 11.0918

>AT30285 WP_023365651.1 NC_022663:1085130-1085327 [Mycobacterium kansasii ATCC 12478]
VPSLNIGFTDEELVAVRDAAAGENLSLRAFVHRAAVVAASDRKRRVAEAAALVAQRSAELNRRLA

Nucleotide

• Length: 198 bp

>AT30285 NC_022663:1085130-1085327 [Mycobacterium kansasii ATCC 12478]
GTGCCATCGCTGAACATCGGTTTCACCGACGAAGAACTTGTTGCCGTTCGCGACGCAGCAGCCGGTGAAAACTTGTCGCT
GCGCGCGTTTGTTCACCGCGCGGCCGTCGTCGCCGCCAGCGATCGCAAGCGACGGGTCGCCGAAGCTGCGGCTCTGGTGG
CTCAGCGCTCCGCGGAGCTGAACCGCCGCCTGGCGTGA

Structures

Toxin

• AlphaFold DB: X7ZN33

Antitoxin

• AlphaFold DB: X7ZMP8