Detailed information of TA system TA030284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1007850..1008463
• Replicon: chromosome
• Accession: NC_022663
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7ZK47
• Locus tag: MKAN_RS04325
• Protein ID: WP_023365532.1
• Coordinates: 1007850..1008239 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MKAN_RS04330
• Protein ID: WP_036393214.1
• Coordinates: 1008236..1008463 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS04300	1003161..1003406	+	246	WP_023365524.1	hypothetical protein	-
MKAN_RS04305	1003413..1005491	+	2079	WP_023365526.1	recombinase family protein	-
MKAN_RS29270	1005504..1006531	-	1028	Protein_872	IS3 family transposase	-
MKAN_RS29275	1006587..1006820	-	234	Protein_873	hypothetical protein	-
MKAN_RS31005	1006730..1007068	-	339	WP_023365530.1	hypothetical protein	-
MKAN_RS04325	1007850..1008239	-	390	WP_023365532.1	PIN domain-containing protein	Toxin
MKAN_RS04330	1008236..1008463	-	228	WP_036393214.1	hypothetical protein	Antitoxin
MKAN_RS04335	1008799..1011429	+	2631	WP_023365536.1	cation-transporting P-type ATPase	-
MKAN_RS04340	1011474..1011701	-	228	WP_023365538.1	hypothetical protein	-
MKAN_RS04345	1011724..1012104	-	381	WP_042313314.1	DUF732 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	993525..1027809	34284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-116)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13970.16 Da
• Isoelectric Point: 7.0619

>T30284 WP_023365532.1 NC_022663:c1008239-1007850 [Mycobacterium kansasii ATCC 12478]
VIVLDASAAVELVLATPPGAAVARRLRDETVHAPAHIDVEVIGAIRRAVVRRLISDHEGVVAVADFLSLQIRRWPIKPFA
QRAYQLRTTHIVADAVYVALAEGFGVRLITCDGRLAQSHGHDAEIELVA

Nucleotide

• Length: 390 bp

>T30284 NC_022663:c1008239-1007850 [Mycobacterium kansasii ATCC 12478]
GTGATTGTGTTGGATGCCTCGGCGGCTGTCGAGCTTGTCCTCGCGACGCCGCCTGGTGCGGCCGTCGCCCGCCGCCTGCG
CGACGAGACCGTGCATGCGCCTGCCCACATCGATGTCGAGGTGATCGGGGCGATACGTCGAGCAGTCGTGCGCCGGCTGA
TCAGCGATCATGAAGGAGTCGTCGCGGTTGCCGACTTTCTAAGCCTGCAGATTCGGCGTTGGCCTATAAAGCCATTCGCC
CAGCGCGCCTATCAACTGCGGACCACTCATATCGTGGCCGACGCAGTTTACGTCGCCCTCGCCGAGGGCTTCGGCGTACG
GCTGATCACTTGCGACGGGCGACTCGCGCAATCCCACGGCCACGATGCAGAGATCGAGCTCGTCGCCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.38 Da
• Isoelectric Point: 4.7513

>AT30284 WP_036393214.1 NC_022663:c1008463-1008236 [Mycobacterium kansasii ATCC 12478]
MSTMVQIRNVPDELVHELKARAAAQRMSLSDFLLARLAEFAQEPALDEVLGRLAALPRRDLGASAAELVGEARSE

Nucleotide

• Length: 228 bp

>AT30284 NC_022663:c1008463-1008236 [Mycobacterium kansasii ATCC 12478]
ATGTCCACTATGGTTCAAATCCGCAACGTCCCTGACGAACTTGTCCACGAGCTGAAGGCCCGTGCAGCGGCGCAGCGAAT
GAGCCTCAGTGATTTTCTGCTGGCACGGCTCGCCGAGTTTGCGCAAGAACCGGCCCTCGACGAGGTTCTCGGCCGTCTCG
CGGCGCTGCCACGGCGGGATCTCGGCGCGAGCGCAGCTGAACTGGTTGGCGAGGCTCGATCCGAGTGA

Structures

Toxin

• AlphaFold DB: X7ZK47