Detailed information of TA system TA030283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3194871..3195526
• Replicon: chromosome
• Accession: NC_022658
• Organism: Burkholderia pseudomallei NCTC 13179

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BBK_RS31120
• Protein ID: WP_041191053.1
• Coordinates: 3195230..3195526 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BBK_RS31115
• Protein ID: WP_004202809.1
• Coordinates: 3194871..3195227 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBK_RS38870	3189894..3191321	-	1428	WP_043280825.1	DNA (cytosine-5-)-methyltransferase	-
BBK_RS31075	3191574..3191792	-	219	WP_023358395.1	hypothetical protein	-
BBK_RS41055	3191949..3192086	-	138	WP_006027274.1	hypothetical protein	-
BBK_RS31090	3192314..3192535	-	222	WP_009897093.1	hypothetical protein	-
BBK_RS31095	3192851..3193633	-	783	WP_009976613.1	hypothetical protein	-
BBK_RS31105	3194014..3194352	+	339	WP_009976611.1	helix-turn-helix domain-containing protein	-
BBK_RS31110	3194678..3194827	+	150	WP_023358778.1	phage portal protein	-
BBK_RS31115	3194871..3195227	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BBK_RS31120	3195230..3195526	-	297	WP_041191053.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBK_RS38885	3196158..3196871	-	714	WP_080002914.1	cellulose biosynthesis protein BcsS	-
BBK_RS31125	3196798..3199047	-	2250	WP_009979045.1	glycosyltransferase	-
BBK_RS31130	3199158..3200525	-	1368	WP_051408745.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3154403..3214999	60596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10825.60 Da
• Isoelectric Point: 9.9377

>T30283 WP_041191053.1 NC_022658:c3195526-3195230 [Burkholderia pseudomallei NCTC 13179]
MFKVLTTPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWRAVGDGVNEMKIDVGPGYRAYFVRHGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T30283 NC_022658:c3195526-3195230 [Burkholderia pseudomallei NCTC 13179]
ATGTTCAAAGTTCTGACGACCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCGCGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACACGGAAAAATTATCGTCGTGGTGTTGTGCGGTGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT30283 WP_004202809.1 NC_022658:c3195227-3194871 [Burkholderia pseudomallei NCTC 13179]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT30283 NC_022658:c3195227-3194871 [Burkholderia pseudomallei NCTC 13179]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAATACCTGAAGGACGAGGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAATCCGCGCCTGATTCAAGCCGCGCTTGGGAACGTTGCGAAGGCGCGCGGCATGACGGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTACCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCAGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCACACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3