Detailed information of TA system TA030282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3182771..3183426
• Replicon: chromosome
• Accession: NC_022658
• Organism: Burkholderia pseudomallei NCTC 13179

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: BBK_RS31020
• Protein ID: WP_038787990.1
• Coordinates: 3183130..3183426 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q63YL3
• Locus tag: BBK_RS31015
• Protein ID: WP_004202809.1
• Coordinates: 3182771..3183127 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BBK_RS38860	3178074..3178670	-	597	WP_080399440.1	hypothetical protein	-
BBK_RS41045	3178744..3179268	-	525	WP_197540008.1	transposase	-
BBK_RS41050	3179275..3179712	-	438	WP_023358690.1	IS630 family transposase	-
BBK_RS31005	3179906..3181675	+	1770	WP_023358772.1	terminase ATPase subunit family protein	-
BBK_RS31010	3181672..3182727	+	1056	WP_023358744.1	phage portal protein	-
BBK_RS31015	3182771..3183127	-	357	WP_004202809.1	putative addiction module antidote protein	Antitoxin
BBK_RS31020	3183130..3183426	-	297	WP_038787990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BBK_RS31025	3184017..3185132	-	1116	WP_038749743.1	DUF3396 domain-containing protein	-
BBK_RS31030	3185147..3185884	-	738	WP_004532838.1	VRR-NUC domain-containing protein	-
BBK_RS31035	3185881..3186414	-	534	WP_004532793.1	bacteriophage gp29 protein	-
BBK_RS31040	3186517..3186639	-	123	WP_009897078.1	CopG family transcriptional regulator	-
BBK_RS31045	3186873..3187526	-	654	WP_004532770.1	AAA family ATPase	-
BBK_RS31050	3187616..3187822	-	207	WP_004532731.1	ogr/Delta-like zinc finger family protein	-
BBK_RS31055	3187819..3188085	-	267	WP_023358427.1	ogr/Delta-like zinc finger family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3154403..3214999	60596
	-	flank	IS/Tn	-	-	3179275..3179712	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (8-97)

Antitoxin

• Uncharacterized protein HI1420 (16-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10821.61 Da
• Isoelectric Point: 9.9377

>T30282 WP_038787990.1 NC_022658:c3183426-3183130 [Burkholderia pseudomallei NCTC 13179]
MFKVLTPPQFDKWLDGLRDPVGSAAINLRIERAKLGNLGQWHAVGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKLAKQIAGELED

Nucleotide

• Length: 297 bp

>T30282 NC_022658:c3183426-3183130 [Burkholderia pseudomallei NCTC 13179]
ATGTTCAAAGTTCTGACGCCCCCCCAGTTTGACAAATGGCTTGACGGGCTTCGCGATCCGGTCGGTAGCGCGGCAATCAA
CCTGCGCATCGAGCGGGCGAAGCTTGGCAATCTCGGCCAGTGGCACGCAGTCGGCGACGGCGTCAACGAAATGAAGATTG
ATGTGGGGCCGGGATATCGGGCCTACTTCGTGCGACGCGGAAAAATTATCGTCGTGGTGTTGTGCGGCGGGGACAAGTCG
ACGCAGAAGAAGGACATCAAGCTAGCGAAGCAAATCGCTGGCGAACTGGAGGATTGA

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 12585.41 Da
• Isoelectric Point: 9.9470

>AT30282 WP_004202809.1 NC_022658:c3183127-3182771 [Burkholderia pseudomallei NCTC 13179]
MKISELAEFDGSKYLKDEETIRHYLAQAFEDGNPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVGALGLHLTVAPAEPAPVPAPATTRARSRVRTAAHA

Nucleotide

• Length: 357 bp

>AT30282 NC_022658:c3183127-3182771 [Burkholderia pseudomallei NCTC 13179]
ATGAAAATCAGCGAACTGGCCGAGTTCGACGGCTCGAAGTACCTGAAGGACGAAGAAACGATTCGTCACTACCTGGCGCA
AGCGTTCGAGGATGGAAATCCGCGCCTGATTCAAGCCGCGCTTGGGAACGTTGCGAAGGCGCGCGGCATGACGGCGCTCG
CGCGCGAGTCCGGCGTGAAGCGTGAAGCGCTCTACCGCGCGCTGTCGGAAGGTGGGAACGCGGAATTCGCAACGATCATG
AAAGTTGTGGGCGCGTTGGGGCTGCACCTGACCGTTGCGCCGGCCGAACCTGCGCCGGTGCCCGCGCCGGCAACAACGCG
TGCACGCTCGCGCGTTCGCACGGCTGCGCACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3N4G1D3