30281

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 2580776..2581313 Replicon chromosome
Accession NC_022658
Organism Burkholderia pseudomallei NCTC 13179

Toxin (Protein)


Gene name brnT Uniprot ID Q2T6G2
Locus tag BBK_RS28670 Protein ID WP_004549731.1
Coordinates 2580776..2581045 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q63NA3
Locus tag BBK_RS28675 Protein ID WP_004525420.1
Coordinates 2581029..2581313 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBK_RS28635 2577307..2577735 - 429 Protein_2190 DDE-type integrase/transposase/recombinase -
BBK_RS41015 2577733..2578446 - 714 Protein_2191 prohibitin family protein -
BBK_RS28645 2578603..2579183 + 581 Protein_2192 hypothetical protein -
BBK_RS28660 2579789..2579920 - 132 WP_009923452.1 bacteriophage protein Gp48 -
BBK_RS28665 2579930..2580205 - 276 WP_009975384.1 hypothetical protein -
BBK_RS28670 2580776..2581045 + 270 WP_004549731.1 BrnT family toxin Toxin
BBK_RS28675 2581029..2581313 + 285 WP_004525420.1 BrnA antitoxin family protein Antitoxin
BBK_RS28685 2581961..2582344 + 384 Protein_2197 DDE-type integrase/transposase/recombinase -
BBK_RS38335 2582307..2582735 + 429 Protein_2198 IS110 family transposase -
BBK_RS28690 2582962..2583078 - 117 Protein_2199 transposase -
BBK_RS28695 2583325..2584785 + 1461 WP_023358159.1 metallophosphoesterase -
BBK_RS28700 2584944..2586206 + 1263 WP_009975402.1 c-type cytochrome -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2574996..2582344 7348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(27-88)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10157.46 Da        Isoelectric Point: 5.6831

>T30281 WP_004549731.1 NC_022658:2580776-2581045 [Burkholderia pseudomallei NCTC 13179]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Download         Length: 270 bp

>T30281 NC_022658:2580776-2581045 [Burkholderia pseudomallei NCTC 13179]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10697.10 Da        Isoelectric Point: 5.8930

>AT30281 WP_004525420.1 NC_022658:2581029-2581313 [Burkholderia pseudomallei NCTC 13179]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Download         Length: 285 bp

>AT30281 NC_022658:2581029-2581313 [Burkholderia pseudomallei NCTC 13179]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAGGCAACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q2T6G2

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6L9S8

References