Detailed information of TA system TA030278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 20671..21589
• Replicon: plasmid pCC42yr
• Accession: NC_022656
• Organism: Campylobacter coli 15-537360

Toxin (Protein)

• Gene name: Fic
• Uniprot ID: -
• Locus tag: N149_RS08545
• Protein ID: WP_023361565.1
• Coordinates: 20906..21589 (+)
• Length: 228 a.a.

Antitoxin (Protein)

• Gene name: Fti
• Uniprot ID: A0A0Q2TTN4
• Locus tag: N149_RS08540
• Protein ID: WP_002804094.1
• Coordinates: 20671..20913 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N149_RS08755	15672..15812	+	141	WP_002779802.1	hypothetical protein	-
N149_RS08525	15817..18030	+	2214	WP_002795783.1	DUF1738 domain-containing protein	-
N149_RS08530	18027..19850	+	1824	WP_023361561.1	type IV secretory system conjugative DNA transfer family protein	-
N149_RS08535	19861..20580	+	720	WP_023361563.1	IncP-type DNA transfer protein TraL	-
N149_RS08540	20671..20913	+	243	WP_002804094.1	hypothetical protein	Antitoxin
N149_RS08545	20906..21589	+	684	WP_023361565.1	Fic family protein	Toxin
N149_RS08550	21594..22061	+	468	WP_002798254.1	signal peptidase I	-
N149_RS08555	22126..22521	+	396	WP_002782959.1	conjugal transfer protein TraM	-
N149_RS08560	22613..23299	+	687	WP_023361567.1	AAA family ATPase	-
N149_RS08565	23302..23526	+	225	WP_002790621.1	hypothetical protein	-
N149_RS08570	23767..25923	-	2157	WP_023361569.1	relaxase/mobilization nuclease domain-containing protein	-
N149_RS08575	25886..26269	-	384	WP_002797386.1	DNA transfer protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26269	26269

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (60-169)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 228 a.a.
• Molecular weight: 25801.78 Da
• Isoelectric Point: 10.1130

>T30278 WP_023361565.1 NC_022656:20906-21589 [Campylobacter coli 15-537360]
MIDKVLKFLDDGIPKNDFDRQLLASNLVGATNLIELNKKERIITLQAAKKLELNPIKGNFDYQHLKDIHKALFKDVYTWA
GKDRMEVGLHGNFGKYAPNGTITNFVPGKDLNVTAQQIFTWLKEDNYLTNSKDLNDFAKNLAEFARNLNALHPFREGNGR
TQRIFLNELAKNAGYKLDLNLIPKNKMIMASVEASQLKLGKLEAIIKTNLKSFRQNLDLEQNKGISL

Nucleotide

• Length: 684 bp

>T30278 NC_022656:20906-21589 [Campylobacter coli 15-537360]
ATGATTGATAAAGTTTTGAAATTTTTAGATGATGGTATTCCTAAAAATGATTTTGATAGACAACTTTTAGCTTCTAATTT
AGTAGGCGCAACAAATTTAATAGAGTTAAATAAAAAAGAAAGAATAATAACTCTACAAGCCGCTAAAAAATTAGAGCTTA
ATCCAATTAAAGGCAATTTTGACTATCAACACTTAAAAGATATACATAAAGCACTTTTTAAAGATGTTTATACTTGGGCT
GGAAAAGATAGAATGGAAGTAGGTTTGCATGGAAATTTTGGAAAATATGCACCAAATGGAACAATTACTAATTTTGTTCC
TGGAAAAGATTTAAATGTAACCGCTCAACAAATTTTTACTTGGCTTAAAGAAGACAATTACTTAACAAATTCAAAAGATC
TTAACGATTTTGCAAAGAACTTAGCCGAATTTGCTAGAAACCTAAATGCTTTGCACCCCTTTAGAGAAGGTAACGGCAGA
ACTCAAAGAATATTTTTAAATGAATTGGCTAAAAATGCAGGTTATAAACTTGATTTAAATTTAATACCAAAAAATAAAAT
GATAATGGCATCTGTAGAAGCTTCACAATTGAAATTAGGAAAACTTGAAGCCATAATAAAAACAAATCTAAAAAGCTTTA
GACAAAACTTAGATTTAGAGCAAAATAAAGGAATATCATTGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9452.68 Da
• Isoelectric Point: 4.3535

>AT30278 WP_002804094.1 NC_022656:20671-20913 [Campylobacter coli 15-537360]
MQKQYPEVHSLEESLAILKKYKDDLTKEQYEQNKSIICGFAIENMFANEEDIINLIKVDKQEKTPDEIIAEYKKEWGVND

Nucleotide

• Length: 243 bp

>AT30278 NC_022656:20671-20913 [Campylobacter coli 15-537360]
ATGCAAAAACAATATCCCGAAGTGCATAGCTTAGAAGAAAGCCTTGCGATACTTAAAAAATATAAAGATGATTTAACTAA
AGAGCAATATGAGCAAAATAAATCTATTATTTGTGGATTTGCTATTGAGAATATGTTTGCAAATGAAGAAGACATTATAA
ATCTCATAAAAGTAGATAAACAAGAAAAAACACCTGATGAAATAATCGCCGAATATAAAAAAGAGTGGGGTGTAAATGAT
TGA

Structures

Antitoxin

• AlphaFold DB: A0A0Q2TTN4