Detailed information of TA system TA030277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/GNAT-DUF1778
• Location: 139558..140402
• Replicon: plasmid pMK12478
• Accession: NC_022654
• Organism: Mycobacterium kansasii ATCC 12478

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: MKAN_RS28500
• Protein ID: WP_086361399.1
• Coordinates: 139944..140402 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MKAN_RS28495
• Protein ID: WP_169733146.1
• Coordinates: 139558..139908 (+)
• Length: 117 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKAN_RS28490	139196..139525	+	330	WP_155254719.1	hypothetical protein	-
MKAN_RS28495	139558..139908	+	351	WP_169733146.1	DUF1778 domain-containing protein	Antitoxin
MKAN_RS28500	139944..140402	+	459	WP_086361399.1	GNAT family N-acetyltransferase	Toxin
MKAN_RS28505	140798..141667	-	870	WP_052622775.1	pyridoxamine 5'-phosphate oxidase family protein	-
MKAN_RS28510	141703..142272	-	570	WP_023363549.1	TIGR03086 family protein	-
MKAN_RS28515	142373..142969	+	597	WP_023363551.1	ABATE domain-containing protein	-
MKAN_RS28520	143159..143791	-	633	WP_023363553.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN	1..144951	144951

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-131)

Antitoxin

• Vibrio phage ICP1, Orf50 (36-109)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16886.44 Da
• Isoelectric Point: 10.2690

>T30277 WP_086361399.1 NC_022654:139944-140402 [Mycobacterium kansasii ATCC 12478]
IVDDFDCGNDVLNEWLRTKALHNQREGGSRTWVVLDGRRVVAFYASSTAVLMRSRATKRAARNQPDPLPAVLLGRLAVDA
NHQGRGLAAALLKHFILKSLEVAELTGVRLLLVHANNDTARRFYLRYGFEPSPVDDLTLMLLVKDVRPSPSA

Nucleotide

• Length: 459 bp

>T30277 NC_022654:139944-140402 [Mycobacterium kansasii ATCC 12478]
ATCGTCGACGACTTCGACTGCGGCAACGACGTGCTCAACGAGTGGCTGCGGACGAAGGCTCTGCACAATCAGCGCGAAGG
AGGCAGCCGCACCTGGGTGGTGCTGGACGGGCGACGGGTGGTGGCCTTCTATGCCTCCAGCACGGCGGTGCTCATGCGCA
GCCGGGCGACGAAACGCGCTGCTCGCAATCAGCCGGACCCGTTACCTGCGGTCCTGCTCGGCCGGCTGGCCGTCGACGCG
AACCATCAAGGGCGTGGTCTCGCTGCAGCACTTCTCAAACATTTCATCCTCAAGTCACTTGAGGTCGCGGAGCTGACGGG
GGTTCGGCTGCTGCTGGTCCACGCAAACAACGACACCGCCCGGCGCTTCTACTTGCGCTACGGTTTTGAGCCTTCTCCTG
TCGACGACCTAACACTGATGCTCCTCGTAAAGGACGTCCGGCCCAGCCCATCTGCGTAA

Antitoxin

Protein

• Length: 117 a.a.
• Molecular weight: 12762.38 Da
• Isoelectric Point: 6.2265

>AT30277 WP_169733146.1 NC_022654:139558-139908 [Mycobacterium kansasii ATCC 12478]
MGTPPRAELHRSALADGMYTNSAYTSGVETKSGRWHLRVTAAQDAVVRRVLDVTGESLNDYVVRHAVQAAEADLADRRVF
VLDDAAWTDLQALLDRPPSPKPELARLLANPSILER

Nucleotide

• Length: 351 bp

>AT30277 NC_022654:139558-139908 [Mycobacterium kansasii ATCC 12478]
TTGGGAACGCCGCCGCGCGCCGAACTGCATCGTTCCGCGCTTGCCGACGGTATGTACACCAATTCGGCGTACACTTCTGG
TGTGGAGACGAAGAGTGGTCGCTGGCACCTGCGCGTGACCGCGGCGCAAGATGCGGTGGTGCGACGTGTGCTCGACGTCA
CGGGCGAGTCGCTCAACGACTATGTGGTGCGTCACGCCGTTCAGGCCGCCGAGGCCGACCTCGCTGATCGGAGAGTGTTC
GTCCTCGATGACGCCGCCTGGACCGATCTTCAGGCGCTGCTCGACCGTCCGCCAAGCCCCAAGCCCGAGTTGGCGCGCCT
GCTGGCGAATCCGTCGATTTTGGAACGGTGA