Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 77546..78228 Replicon plasmid pMK12478
Accession NC_022654
Organism Mycobacterium kansasii ATCC 12478

Toxin (Protein)


Gene name vapC Uniprot ID A0A854IDQ2
Locus tag MKAN_RS28190 Protein ID WP_040624374.1
Coordinates 77812..78228 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID D5P579
Locus tag MKAN_RS28185 Protein ID WP_007172349.1
Coordinates 77546..77809 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MKAN_RS28160 72618..74063 - 1446 WP_101930849.1 type VII secretion protein EccB -
MKAN_RS28165 74177..75217 - 1041 WP_023363502.1 C40 family peptidase -
MKAN_RS29025 75303..76406 - 1104 WP_051404593.1 hypothetical protein -
MKAN_RS28180 76470..77444 - 975 WP_023363505.1 hypothetical protein -
MKAN_RS28185 77546..77809 + 264 WP_007172349.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MKAN_RS28190 77812..78228 + 417 WP_040624374.1 type II toxin-antitoxin system VapC family toxin Toxin
MKAN_RS28195 78510..79772 - 1263 WP_169733147.1 IS256 family transposase -
MKAN_RS28200 79984..80565 + 582 WP_007172352.1 IS607 family transposase -
MKAN_RS28205 80565..82217 + 1653 WP_040624340.1 IS607 family element transposase accessory protein TnpB -
MKAN_RS29030 82323..82461 + 139 Protein_86 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - eccA5 / esxN 1..144951 144951


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(1-82)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14879.84 Da        Isoelectric Point: 5.4519

>T30274 WP_040624374.1 NC_022654:77812-78228 [Mycobacterium kansasii ATCC 12478]
MIIDSSAIVALIQGEDPYTEQIAAAIAADPSPVMSTANAAECLIVLTNRHGATARTVFDRLRSEINLGFQPFTLEHAIAA
HRAYLHYGKGRHPAALNYGDTMAYATAKLAHEPLIAIGNDFTQTDLEFNGVIGYWPAP

Download         Length: 417 bp

>T30274 NC_022654:77812-78228 [Mycobacterium kansasii ATCC 12478]
CTGATCATCGACTCTTCCGCGATCGTCGCCCTCATCCAGGGCGAGGACCCCTACACCGAACAGATCGCGGCCGCCATCGC
CGCCGATCCCAGCCCCGTCATGTCCACGGCCAACGCAGCCGAATGCCTCATCGTTCTCACCAACCGACACGGCGCGACCG
CGCGCACCGTCTTCGACCGCCTACGATCCGAAATCAACCTCGGCTTCCAGCCCTTCACCCTCGAACACGCGATCGCCGCC
CACCGCGCCTACCTCCACTACGGAAAAGGCCGCCACCCCGCCGCCCTCAACTACGGCGACACCATGGCCTACGCCACCGC
CAAACTCGCACACGAACCCCTCATCGCCATCGGCAACGACTTCACCCAAACCGACCTCGAATTCAACGGCGTCATCGGCT
ACTGGCCAGCGCCCTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9884.20 Da        Isoelectric Point: 4.8359

>AT30274 WP_007172349.1 NC_022654:77546-77809 [Mycobacterium kansasii ATCC 12478]
MAFNVKDEEVIRFADELAARLHLPSRIDAIRYALRAQIEVTQSRTANRSAELLDVLKTEIWPLLDDRSPITKTEREQALG
YDTATGV

Download         Length: 264 bp

>AT30274 NC_022654:77546-77809 [Mycobacterium kansasii ATCC 12478]
ATGGCTTTCAACGTGAAAGACGAAGAGGTCATCCGCTTCGCAGACGAGCTCGCCGCGCGCCTCCACCTCCCCAGCCGCAT
CGACGCCATCCGCTACGCCCTGCGAGCCCAGATCGAAGTCACCCAATCCCGCACCGCAAACCGCTCCGCAGAACTGCTCG
ACGTCCTCAAAACCGAGATCTGGCCACTACTGGACGACCGATCCCCAATCACCAAAACCGAACGCGAACAAGCCCTCGGA
TACGACACAGCAACGGGAGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A854IDQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y0TBQ2

References