Detailed information of TA system TA030272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 488907..489544
• Replicon: chromosome
• Accession: NC_022653
• Organism: Bacillus amyloliquefaciens CC178

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: U471_RS02475
• Protein ID: WP_003156187.1
• Coordinates: 489194..489544 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: U471_RS02470
• Protein ID: WP_003156188.1
• Coordinates: 488907..489188 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
U471_RS02450	485272..485871	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
U471_RS02455	485964..486329	+	366	WP_012116869.1	holo-ACP synthase	-
U471_RS02460	486494..487501	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
U471_RS02465	487618..488787	+	1170	WP_012116870.1	alanine racemase	-
U471_RS02470	488907..489188	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
U471_RS02475	489194..489544	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
U471_RS02480	489662..490483	+	822	WP_003156182.1	STAS domain-containing protein	-
U471_RS02485	490488..490853	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
U471_RS02490	490856..491257	+	402	WP_003156178.1	anti-sigma regulatory factor	-
U471_RS02495	491269..492276	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
U471_RS02500	492340..492669	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
U471_RS02505	492666..493148	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
U471_RS02510	493114..493902	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
U471_RS02515	493902..494504	+	603	WP_012116871.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T30272 WP_003156187.1 NC_022653:489194-489544 [Bacillus amyloliquefaciens CC178]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T30272 NC_022653:489194-489544 [Bacillus amyloliquefaciens CC178]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT30272 WP_003156188.1 NC_022653:488907-489188 [Bacillus amyloliquefaciens CC178]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT30272 NC_022653:488907-489188 [Bacillus amyloliquefaciens CC178]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5