Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 105134..105777 Replicon plasmid pJJ1886_5
Accession NC_022651
Organism Escherichia coli JJ1886

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag P423_RS26955 Protein ID WP_001034046.1
Coordinates 105134..105550 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag P423_RS26960 Protein ID WP_001261278.1
Coordinates 105547..105777 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P423_RS26940 100271..100687 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
P423_RS26945 100684..100914 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
P423_RS26950 101295..105089 + 3795 WP_001144732.1 hypothetical protein -
P423_RS26955 105134..105550 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
P423_RS26960 105547..105777 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
P423_RS26965 106042..106542 + 501 WP_000528932.1 hypothetical protein -
P423_RS26970 106555..107328 + 774 WP_000905949.1 hypothetical protein -
P423_RS26975 107539..109152 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
P423_RS26980 109183..109533 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
P423_RS26985 109530..109955 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..110040 110040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T30271 WP_001034046.1 NC_022651:c105550-105134 [Escherichia coli JJ1886]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T30271 NC_022651:c105550-105134 [Escherichia coli JJ1886]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT30271 WP_001261278.1 NC_022651:c105777-105547 [Escherichia coli JJ1886]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT30271 NC_022651:c105777-105547 [Escherichia coli JJ1886]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References