Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 93255..93814 Replicon plasmid pJJ1886_5
Accession NC_022651
Organism Escherichia coli JJ1886

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag P423_RS26890 Protein ID WP_000421272.1
Coordinates 93539..93814 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag P423_RS26885 Protein ID WP_001387467.1
Coordinates 93255..93539 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P423_RS26855 88718..89158 - 441 Protein_106 zinc-binding dehydrogenase -
P423_RS26865 89559..90449 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
P423_RS26870 90474..90854 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
P423_RS26875 90887..91852 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
P423_RS26880 91898..92650 - 753 WP_000562172.1 IclR family transcriptional regulator -
P423_RS26885 93255..93539 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
P423_RS26890 93539..93814 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
P423_RS26895 93920..94213 + 294 WP_001105060.1 hypothetical protein -
P423_RS30310 94248..94376 - 129 Protein_114 IS481 family transposase -
P423_RS26900 94409..95578 - 1170 WP_001072355.1 hypothetical protein -
P423_RS26910 96071..96378 + 308 Protein_116 type II toxin-antitoxin system ParD family antitoxin -
P423_RS26920 96667..97364 + 698 Protein_117 IS1 family transposase -
P423_RS26930 97618..98640 - 1023 WP_000361402.1 helicase UvrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..110040 110040
- flank IS/Tn - - 96861..97364 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T30269 WP_000421272.1 NC_022651:93539-93814 [Escherichia coli JJ1886]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T30269 NC_022651:93539-93814 [Escherichia coli JJ1886]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT30269 WP_001387467.1 NC_022651:93255-93539 [Escherichia coli JJ1886]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT30269 NC_022651:93255-93539 [Escherichia coli JJ1886]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References