Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4752..5277 Replicon plasmid pJJ1886_5
Accession NC_022651
Organism Escherichia coli JJ1886

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag P423_RS26330 Protein ID WP_001159868.1
Coordinates 4972..5277 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag P423_RS26325 Protein ID WP_000813634.1
Coordinates 4752..4970 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P423_RS26300 31..1644 - 1614 WP_000080195.1 IS66-like element ISEc23 family transposase -
P423_RS26305 1675..2025 - 351 WP_000624722.1 IS66 family insertion sequence element accessory protein TnpB -
P423_RS26310 2022..2447 - 426 WP_000422741.1 IS66 family insertion sequence hypothetical protein -
P423_RS26315 2509..3660 + 1152 WP_031325939.1 DUF3800 domain-containing protein -
P423_RS26320 3694..4206 - 513 WP_000151784.1 hypothetical protein -
P423_RS26325 4752..4970 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
P423_RS26330 4972..5277 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
P423_RS26335 5278..6084 + 807 WP_000016982.1 site-specific integrase -
P423_RS26340 6858..7613 + 756 WP_000852146.1 replication initiation protein RepE -
P423_RS26345 8201..9367 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B - 1..110040 110040


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T30264 WP_001159868.1 NC_022651:4972-5277 [Escherichia coli JJ1886]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T30264 NC_022651:4972-5277 [Escherichia coli JJ1886]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT30264 WP_000813634.1 NC_022651:4752-4970 [Escherichia coli JJ1886]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT30264 NC_022651:4752-4970 [Escherichia coli JJ1886]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References