Detailed information of TA system TA030261

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4992226..4993061
• Replicon: chromosome
• Accession: NC_022648
• Organism: Escherichia coli JJ1886

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J2AEA6
• Locus tag: P423_RS25120
• Protein ID: WP_000854759.1
• Coordinates: 4992684..4993061 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: P423_RS25115
• Protein ID: WP_001295723.1
• Coordinates: 4992226..4992594 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P423_RS25085	4989341..4989499	+	159	WP_001323397.1	DUF905 family protein	-
P423_RS25090	4989654..4990472	+	819	WP_001234738.1	DUF945 domain-containing protein	-
P423_RS25095	4990814..4991287	+	474	WP_001350782.1	antirestriction protein	-
P423_RS25100	4991303..4991779	+	477	WP_001186775.1	RadC family protein	-
P423_RS25105	4991842..4992063	+	222	WP_000692345.1	DUF987 domain-containing protein	-
P423_RS25115	4992226..4992594	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
P423_RS25120	4992684..4993061	+	378	WP_000854759.1	TA system toxin CbtA family protein	Toxin
P423_RS25125	4993058..4993546	+	489	WP_000761690.1	hypothetical protein	-
P423_RS25130	4993563..4993739	+	177	WP_000839286.1	DUF957 domain-containing protein	-
P423_RS28880	4993845..4994042	+	198	WP_144079987.1	hypothetical protein	-
P423_RS25135	4994057..4994482	+	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
P423_RS25140	4994479..4994829	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
P423_RS25145	4994860..4996473	+	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
P423_RS30230	4996900..4997076	+	177	Protein_4750	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14144.26 Da
• Isoelectric Point: 7.3249

>T30261 WP_000854759.1 NC_022648:4992684-4993061 [Escherichia coli JJ1886]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T30261 NC_022648:4992684-4993061 [Escherichia coli JJ1886]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT30261 WP_001295723.1 NC_022648:4992226-4992594 [Escherichia coli JJ1886]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT30261 NC_022648:4992226-4992594 [Escherichia coli JJ1886]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J2AEA6

Antitoxin

• AlphaFold DB: S1NM52