Detailed information of TA system TA030222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2284165..2284694
• Replicon: chromosome
• Accession: NC_022604
• Organism: Staphylococcus aureus subsp. aureus Z172

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SAZ172_RS11830
• Protein ID: WP_000621175.1
• Coordinates: 2284165..2284527 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SAZ172_RS11835
• Protein ID: WP_000948331.1
• Coordinates: 2284524..2284694 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAZ172_RS11805	2281144..2281914	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
SAZ172_RS11810	2281889..2282368	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
SAZ172_RS11815	2282370..2282696	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SAZ172_RS11820	2282815..2283816	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SAZ172_RS11830	2284165..2284527	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SAZ172_RS11835	2284524..2284694	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SAZ172_RS11840	2284779..2285927	-	1149	WP_001281145.1	alanine racemase	-
SAZ172_RS11845	2285993..2286352	-	360	WP_000581200.1	holo-ACP synthase	-
SAZ172_RS11850	2286356..2286847	-	492	WP_001205910.1	PH domain-containing protein	-
SAZ172_RS11855	2286834..2288417	-	1584	WP_001294626.1	PH domain-containing protein	-
SAZ172_RS11860	2288410..2288889	-	480	WP_001287088.1	hypothetical protein	-
SAZ172_RS11865	2289097..2289657	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T30222 WP_000621175.1 NC_022604:c2284527-2284165 [Staphylococcus aureus subsp. aureus Z172]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T30222 NC_022604:c2284527-2284165 [Staphylococcus aureus subsp. aureus Z172]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT30222 WP_000948331.1 NC_022604:c2284694-2284524 [Staphylococcus aureus subsp. aureus Z172]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT30222 NC_022604:c2284694-2284524 [Staphylococcus aureus subsp. aureus Z172]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1