Detailed information of TA system TA030211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-shpB/BrnT-BrnA
• Location: 3129231..3129786
• Replicon: chromosome
• Accession: NC_022600
• Organism: Gloeobacter kilaueensis JS1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: GKIL_RS14445
• Protein ID: WP_041244805.1
• Coordinates: 3129496..3129786 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: U5QJU2
• Locus tag: GKIL_RS14440
• Protein ID: WP_023174431.1
• Coordinates: 3129231..3129515 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKIL_RS14425	3124701..3126368	+	1668	WP_023174428.1	FAD-dependent oxidoreductase	-
GKIL_RS14430	3126368..3127750	+	1383	WP_023174429.1	cyclic nucleotide-binding domain-containing protein	-
GKIL_RS14435	3127763..3129196	+	1434	WP_023174430.1	cyclic nucleotide-binding domain-containing protein	-
GKIL_RS14440	3129231..3129515	-	285	WP_023174431.1	BrnA antitoxin family protein	Antitoxin
GKIL_RS14445	3129496..3129786	-	291	WP_041244805.1	BrnT family toxin	Toxin
GKIL_RS14450	3130013..3130408	+	396	WP_023174433.1	hypothetical protein	-
GKIL_RS14455	3130378..3130953	-	576	WP_041244806.1	GNAT family N-acetyltransferase	-
GKIL_RS14460	3131310..3132395	+	1086	WP_023174435.1	3-dehydroquinate synthase	-
GKIL_RS14465	3132402..3133136	-	735	WP_023174436.1	pentapeptide repeat-containing protein	-
GKIL_RS14470	3133193..3134470	-	1278	WP_023174437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-87)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (33-89)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11202.37 Da
• Isoelectric Point: 4.5452

>T30211 WP_041244805.1 NC_022600:c3129786-3129496 [Gloeobacter kilaueensis JS1]
MAWTWDNEKNRTNKCDHGLSFESAQYVFRDLLAVSRPDPYPHEERWQTIGMIGNMTIFVVHTWPDPDPDTGEETGRIISA
REATPYERRAYEEGTL

Nucleotide

• Length: 291 bp

>T30211 NC_022600:c3129786-3129496 [Gloeobacter kilaueensis JS1]
TTGGCGTGGACCTGGGACAATGAGAAGAACCGAACCAATAAGTGCGATCACGGCTTAAGTTTTGAAAGTGCACAGTATGT
TTTCCGCGATCTTTTGGCGGTGAGTCGCCCTGATCCATACCCACACGAGGAGCGCTGGCAGACCATTGGCATGATCGGCA
ACATGACTATTTTCGTCGTGCATACCTGGCCGGACCCTGATCCGGATACTGGTGAAGAGACAGGCAGAATCATTAGTGCC
CGCGAGGCAACTCCTTATGAGCGGAGGGCTTATGAAGAAGGAACACTCTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10835.27 Da
• Isoelectric Point: 7.3175

>AT30211 WP_023174431.1 NC_022600:c3129515-3129231 [Gloeobacter kilaueensis JS1]
MKKEHSNPVPPEVQAEIDALAAADRPIRTDLIPEATGDWSAARRGLFYRPIKQQITLRLDADLIDWFKRNHLQGEGYQTS
INRALREYIAQHQL

Nucleotide

• Length: 285 bp

>AT30211 NC_022600:c3129515-3129231 [Gloeobacter kilaueensis JS1]
ATGAAGAAGGAACACTCTAACCCTGTGCCGCCAGAAGTACAGGCGGAGATCGATGCCCTGGCGGCAGCGGACAGACCTAT
CCGCACCGATCTCATCCCAGAGGCGACTGGAGACTGGAGCGCTGCCCGGCGCGGCTTGTTTTATCGCCCGATCAAGCAGC
AGATTACGCTGCGGCTCGACGCCGATCTGATCGACTGGTTCAAACGCAACCATCTTCAAGGTGAGGGCTACCAGACCAGC
ATCAACCGGGCGCTGCGGGAATATATCGCCCAGCATCAGCTGTAA

Structures

Antitoxin

• AlphaFold DB: U5QJU2