Detailed information of TA system TA030210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2991103..2991899
• Replicon: chromosome
• Accession: NC_022600
• Organism: Gloeobacter kilaueensis JS1

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: U5QJB8
• Locus tag: GKIL_RS13870
• Protein ID: WP_023174296.1
• Coordinates: 2991378..2991899 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: U5QJI1
• Locus tag: GKIL_RS13865
• Protein ID: WP_023174294.1
• Coordinates: 2991103..2991381 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKIL_RS13845	2986236..2987156	-	921	WP_023174290.1	HPr kinase	-
GKIL_RS25315	2987183..2987320	-	138	WP_023174291.1	hypothetical protein	-
GKIL_RS13850	2987714..2988742	-	1029	WP_023171280.1	IS110 family transposase	-
GKIL_RS13855	2989236..2989430	+	195	WP_023174292.1	hypothetical protein	-
GKIL_RS13860	2989439..2990917	-	1479	WP_023174293.1	sugar ABC transporter ATP-binding protein	-
GKIL_RS13865	2991103..2991381	+	279	WP_023174294.1	DUF1778 domain-containing protein	Antitoxin
GKIL_RS13870	2991378..2991899	+	522	WP_023174296.1	GNAT family N-acetyltransferase	Toxin
GKIL_RS13875	2991922..2992974	+	1053	WP_023174297.1	saccharopine dehydrogenase NADP-binding domain-containing protein	-
GKIL_RS13880	2992975..2993430	-	456	WP_041243954.1	hypothetical protein	-
GKIL_RS13885	2993468..2995894	-	2427	WP_023174299.1	sucrose synthase	-
GKIL_RS13890	2995995..2996876	+	882	WP_023174300.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (5-89)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 18571.33 Da
• Isoelectric Point: 8.5291

>T30210 WP_023174296.1 NC_022600:2991378-2991899 [Gloeobacter kilaueensis JS1]
MTPAWHEEAIARHHDRKAFDCGDQDLNNFLRHSARQSHDRGGAKTFLAIGDSDGSILGFYSVAPAELAYARTPEVVRRGL
AKHAVPGFRLARLAVHVDVQKKGLGGQLLLAAGRRCLRTAAEVGGVVLLIDAKSPPAASWYAGFGAVALEDAPLTLMLPL
ETIEISLRAAEKL

Nucleotide

• Length: 522 bp

>T30210 NC_022600:2991378-2991899 [Gloeobacter kilaueensis JS1]
ATGACGCCTGCATGGCACGAGGAAGCGATCGCCAGACACCACGACCGCAAGGCATTTGATTGCGGCGACCAAGATCTCAA
CAACTTCCTGCGCCATTCAGCGCGCCAGAGTCACGACAGGGGCGGTGCAAAAACCTTCCTGGCTATCGGCGACAGCGATG
GGTCGATCCTTGGCTTCTACAGCGTTGCACCGGCAGAGCTTGCCTACGCCCGGACACCGGAAGTTGTCCGTCGTGGGTTG
GCAAAGCACGCAGTACCGGGCTTTCGGCTCGCACGGCTTGCCGTGCATGTCGATGTGCAGAAAAAGGGCCTGGGCGGTCA
GCTTTTACTTGCCGCTGGCCGCCGTTGCCTGCGGACTGCAGCAGAAGTTGGCGGTGTCGTACTCTTGATCGATGCCAAGA
GTCCGCCCGCTGCAAGCTGGTATGCCGGCTTTGGTGCGGTGGCCCTGGAAGATGCACCTCTGACCCTCATGTTGCCGCTC
GAAACGATCGAGATATCCCTGCGAGCTGCAGAAAAGCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10087.75 Da
• Isoelectric Point: 11.3980

>AT30210 WP_023174294.1 NC_022600:2991103-2991381 [Gloeobacter kilaueensis JS1]
MSRAAINDNGRMSFRVRPEQKAILMRAAAIKKTGLTDFVLQNALQAAERVIEAAERVQLSERDSLRVLDLLENPPAPNAK
LRAAAAALPPLR

Nucleotide

• Length: 279 bp

>AT30210 NC_022600:2991103-2991381 [Gloeobacter kilaueensis JS1]
ATGTCCAGAGCTGCAATCAATGACAATGGACGGATGAGCTTCCGGGTGCGGCCTGAGCAGAAGGCGATCCTCATGCGGGC
GGCAGCGATCAAAAAGACTGGTCTGACCGATTTTGTGCTGCAGAATGCCCTACAGGCCGCCGAGAGGGTGATCGAAGCAG
CCGAGCGCGTGCAGCTTTCCGAACGAGACAGTCTGCGAGTTCTCGATCTTCTCGAAAACCCACCGGCACCGAATGCGAAG
CTGCGGGCAGCTGCGGCTGCACTCCCGCCCTTAAGATGA

Structures

Toxin

• AlphaFold DB: U5QJB8

Antitoxin

• AlphaFold DB: U5QJI1