Detailed information of TA system TA030209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazE-vapB/MazF-VagC
• Location: 1289142..1289758
• Replicon: chromosome
• Accession: NC_022600
• Organism: Gloeobacter kilaueensis JS1

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GKIL_RS06190
• Protein ID: WP_071824787.1
• Coordinates: 1289432..1289758 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U5QEX8
• Locus tag: GKIL_RS06185
• Protein ID: WP_023172603.1
• Coordinates: 1289142..1289375 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKIL_RS06170	1284995..1285636	+	642	WP_023172598.1	NYN domain-containing protein	-
GKIL_RS06175	1285637..1286527	+	891	WP_023172599.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
GKIL_RS23390	1286935..1288386	+	1452	WP_023172600.1	AAA family ATPase	-
GKIL_RS23395	1288427..1288888	+	462	WP_023172601.1	endonuclease	-
GKIL_RS06185	1289142..1289375	-	234	WP_023172603.1	virulence-associated protein VapB	Antitoxin
GKIL_RS06190	1289432..1289758	-	327	WP_071824787.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GKIL_RS06195	1290158..1290574	-	417	WP_023172606.1	hypothetical protein	-
GKIL_RS06200	1290685..1291800	-	1116	WP_023172608.1	DNA polymerase III subunit beta	-
GKIL_RS06205	1292218..1293189	+	972	WP_144080333.1	FG-GAP repeat protein	-
GKIL_RS06210	1293198..1293968	-	771	WP_051382697.1	DUF2993 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12244.20 Da
• Isoelectric Point: 7.1337

>T30209 WP_071824787.1 NC_022600:c1289758-1289432 [Gloeobacter kilaueensis JS1]
VAVVNRFDIYLVALDPTRGSEMQKTRPCVVISPNELHQNLRTAIIAPMTTALRSYPTRIDLTFDGKDGQIALDQIRAVDK
SRLLKHLGSLEEETARQICDLLVKMFEY

Nucleotide

• Length: 327 bp

>T30209 NC_022600:c1289758-1289432 [Gloeobacter kilaueensis JS1]
GTGGCAGTGGTAAATCGTTTTGACATCTACCTTGTTGCCCTTGATCCAACGCGAGGTTCTGAGATGCAAAAAACGCGCCC
CTGCGTCGTCATTTCTCCCAACGAACTGCATCAGAATCTACGCACCGCAATCATCGCGCCGATGACAACAGCTTTGCGCT
CCTACCCGACCCGAATTGACCTCACCTTTGACGGGAAGGATGGGCAGATCGCCCTCGATCAAATTCGAGCAGTCGATAAG
TCTCGCTTGCTCAAGCACCTTGGCTCCCTTGAAGAGGAGACAGCTCGACAGATCTGCGACTTGTTGGTGAAGATGTTTGA
GTATTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8762.21 Da
• Isoelectric Point: 4.6386

>AT30209 WP_023172603.1 NC_022600:c1289375-1289142 [Gloeobacter kilaueensis JS1]
VFPERRVKLFKNGRNQVVRIPPEFEPPGEDAIMRKEGERLIIEPTLPKSLLALLVTLAPIDEEFPPSLDPHPEPVEL

Nucleotide

• Length: 234 bp

>AT30209 NC_022600:c1289375-1289142 [Gloeobacter kilaueensis JS1]
GTGTTTCCTGAACGTCGCGTCAAGCTTTTCAAGAACGGACGGAATCAGGTCGTGCGAATTCCCCCCGAATTCGAGCCTCC
AGGCGAGGATGCAATCATGCGCAAGGAAGGCGAGCGGCTGATTATCGAACCGACGTTACCCAAATCATTGCTGGCCCTGC
TGGTAACCCTGGCTCCGATCGACGAAGAGTTTCCGCCAAGCCTCGATCCACATCCAGAACCCGTCGAGCTTTGA

Structures

Antitoxin

• AlphaFold DB: U5QEX8