Detailed information of TA system TA030208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /BrnT(toxin)
• Location: 80628..81117
• Replicon: chromosome
• Accession: NC_022600
• Organism: Gloeobacter kilaueensis JS1

Toxin (Protein)

• Gene name: -
• Uniprot ID: U5QBS7
• Locus tag: GKIL_RS00365
• Protein ID: WP_023171314.1
• Coordinates: 80854..81117 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: U5QBW1
• Locus tag: GKIL_RS00360
• Protein ID: WP_023171313.1
• Coordinates: 80628..80867 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GKIL_RS24375	75716..76876	+	1161	WP_023171305.1	hypothetical protein	-
GKIL_RS00330	76896..77516	-	621	WP_023171306.1	Holliday junction branch migration protein RuvA	-
GKIL_RS00335	77535..78071	-	537	WP_023171307.1	CPBP family intramembrane metalloprotease	-
GKIL_RS00340	78275..78475	+	201	WP_023171308.1	DUF2997 domain-containing protein	-
GKIL_RS00345	78503..78889	+	387	WP_023171309.1	DUF1257 domain-containing protein	-
GKIL_RS00350	78889..79335	+	447	WP_023171310.1	ferredoxin	-
GKIL_RS25095	79428..79601	+	174	WP_023171311.1	hypothetical protein	-
GKIL_RS00355	79598..79822	+	225	WP_041243617.1	hypothetical protein	-
GKIL_RS24380	79929..80330	+	402	WP_144080280.1	hypothetical protein	-
GKIL_RS00360	80628..80867	-	240	WP_023171313.1	hypothetical protein	Antitoxin
GKIL_RS00365	80854..81117	-	264	WP_023171314.1	BrnT family toxin	Toxin
GKIL_RS00370	81260..81733	+	474	WP_023171315.1	VOC family protein	-
GKIL_RS00375	81806..82498	-	693	WP_023171317.1	hypothetical protein	-
GKIL_RS00380	82483..83514	-	1032	WP_023171318.1	FAD-dependent oxidoreductase	-
GKIL_RS00385	83585..83842	+	258	WP_023171319.1	NifU family protein	-
GKIL_RS00390	83907..84218	-	312	WP_023171320.1	hypothetical protein	-
GKIL_RS00395	84376..85335	+	960	WP_041243618.1	aspartate carbamoyltransferase catalytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	73615..81733	8118

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10140.48 Da
• Isoelectric Point: 4.7010

>T30208 WP_023171314.1 NC_022600:c81117-80854 [Gloeobacter kilaueensis JS1]
MEFEFDDAKSAANKIKHGISFDEAQAIWEDSAYLEIPAKTIDEPRNMIIGLIGNKHWSAVITYRDDRIRLISVRRSRIEE
IELYESI

Nucleotide

• Length: 264 bp

>T30208 NC_022600:c81117-80854 [Gloeobacter kilaueensis JS1]
ATGGAGTTTGAATTCGACGATGCCAAAAGCGCGGCAAACAAGATCAAGCATGGGATTTCCTTCGATGAGGCACAAGCCAT
CTGGGAAGATTCCGCCTATCTTGAGATTCCAGCAAAAACGATAGACGAGCCCCGTAATATGATCATTGGCTTAATCGGTA
ACAAACACTGGTCAGCCGTAATCACCTACCGCGATGACCGCATCCGCTTGATCTCGGTGAGACGTTCAAGAATTGAGGAG
ATAGAACTTTATGAAAGCATCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8977.27 Da
• Isoelectric Point: 8.7688

>AT30208 WP_023171313.1 NC_022600:c80867-80628 [Gloeobacter kilaueensis JS1]
MKASDFDQHFDDTDQDMTAMLDISKASRPGLRTKRVNVDFPDWMVKSLDREANRLGVTRQSLIKLWIAEGLKSRGLVGP

Nucleotide

• Length: 240 bp

>AT30208 NC_022600:c80867-80628 [Gloeobacter kilaueensis JS1]
ATGAAAGCATCTGATTTTGACCAGCATTTTGATGATACTGATCAGGACATGACGGCCATGCTTGATATCAGTAAAGCGAG
CCGTCCAGGACTCCGCACAAAACGGGTCAATGTAGATTTTCCCGATTGGATGGTGAAGTCTCTTGATAGAGAAGCAAACC
GCTTGGGTGTTACGCGCCAGTCTCTTATCAAGCTTTGGATCGCAGAGGGCTTGAAGAGCAGGGGTCTAGTCGGCCCGTAG

Structures

Toxin

• AlphaFold DB: U5QBS7

Antitoxin

• AlphaFold DB: U5QBW1