Detailed information of TA system TA030205

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2272662..2273229
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: CENROD_RS09330
• Protein ID: WP_022775116.1
• Coordinates: 2272662..2272928 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: U5N996
• Locus tag: CENROD_RS09335
• Protein ID: WP_022775119.1
• Coordinates: 2272930..2273229 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS09315	2269747..2270373	-	627	WP_022775106.1	nucleotide exchange factor GrpE	-
CENROD_RS09320	2270502..2272079	+	1578	WP_022775109.1	ATP-binding protein	-
CENROD_RS09325	2272076..2272660	+	585	WP_022775113.1	FAD-binding oxidoreductase	-
CENROD_RS09330	2272662..2272928	+	267	WP_022775116.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CENROD_RS09335	2272930..2273229	+	300	WP_022775119.1	putative addiction module antidote protein	Antitoxin
CENROD_RS09340	2273287..2274840	+	1554	WP_022775122.1	ATP-binding protein	-
CENROD_RS14105	2275097..2275300	+	204	WP_202961197.1	hypothetical protein	-
CENROD_RS12610	2275303..2275572	+	270	WP_022775128.1	class I SAM-dependent methyltransferase	-
CENROD_RS13635	2275615..2276058	+	444	WP_022775131.1	hypothetical protein	-
CENROD_RS09355	2276074..2277195	+	1122	WP_041194544.1	DegT/DnrJ/EryC1/StrS family aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2277400..2278233	833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (15-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-92)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 9558.89 Da
• Isoelectric Point: 7.1327

>T30205 WP_022775116.1 NC_022576:2272662-2272928 [Candidatus Symbiobacter mobilis CR]
MVFAICDSPNMNSRRIHARIDRAEDGNFGDCGPVGEGVSEMRIHYGPGYRVYFAQRGMELVILLAGGGKSTQSKDIKTAL
ELARELGG

Nucleotide

• Length: 267 bp

>T30205 NC_022576:2272662-2272928 [Candidatus Symbiobacter mobilis CR]
ATGGTTTTTGCGATTTGTGATTCGCCCAACATGAACTCCCGCCGCATCCATGCCCGCATCGACCGTGCGGAAGATGGAAA
CTTCGGCGACTGTGGACCGGTTGGTGAAGGTGTATCGGAGATGCGGATTCACTATGGGCCTGGCTATCGTGTGTATTTCG
CGCAGCGCGGAATGGAACTGGTGATATTGCTGGCTGGTGGGGGCAAATCTACCCAGTCGAAAGATATCAAAACTGCGCTG
GAACTTGCGCGGGAACTAGGAGGCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10688.31 Da
• Isoelectric Point: 5.9645

>AT30205 WP_022775119.1 NC_022576:2272930-2273229 [Candidatus Symbiobacter mobilis CR]
MGTLKLRKWDSAEHLKTQEDIVLYLEACMEEAGDDAAFIVKALGNIARAKGMTQLANESGLGHESLYTALSGEGNPSFAT
ILKVMHALGLKLHPEIAHV

Nucleotide

• Length: 300 bp

>AT30205 NC_022576:2272930-2273229 [Candidatus Symbiobacter mobilis CR]
ATGGGAACGCTCAAACTGCGCAAATGGGATAGTGCAGAACACCTCAAGACTCAGGAAGATATAGTGTTGTACTTGGAAGC
ATGCATGGAAGAGGCGGGGGACGACGCAGCCTTCATTGTCAAGGCACTTGGTAACATTGCGCGCGCCAAAGGCATGACGC
AACTTGCCAATGAAAGTGGTCTTGGTCACGAAAGTCTGTACACAGCACTCTCTGGCGAAGGCAACCCAAGTTTTGCCACC
ATCCTCAAGGTCATGCATGCGCTTGGTCTCAAGCTTCACCCTGAAATCGCACATGTCTGA

Structures

Antitoxin

• AlphaFold DB: U5N996