Detailed information of TA system TA030204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagD-vapB/VapC-VagC
• Location: 2265700..2266336
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: U5ND13
• Locus tag: CENROD_RS09295
• Protein ID: WP_022775091.1
• Coordinates: 2265700..2266101 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U5ND78
• Locus tag: CENROD_RS09300
• Protein ID: WP_022775095.1
• Coordinates: 2266085..2266336 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS09280	2261472..2264216	-	2745	WP_022775079.1	preprotein translocase subunit SecA	-
CENROD_RS09285	2264391..2265311	-	921	WP_022775083.1	M23 family metallopeptidase	-
CENROD_RS09290	2265329..2265631	+	303	WP_022775087.1	hypothetical protein	-
CENROD_RS09295	2265700..2266101	-	402	WP_022775091.1	type II toxin-antitoxin system VapC family toxin	Toxin
CENROD_RS09300	2266085..2266336	-	252	WP_022775095.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CENROD_RS09305	2266385..2267515	-	1131	WP_022775099.1	molecular chaperone DnaJ	-
CENROD_RS09310	2267623..2269566	-	1944	WP_022775103.1	molecular chaperone DnaK	-
CENROD_RS09315	2269747..2270373	-	627	WP_022775106.1	nucleotide exchange factor GrpE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (8-55)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15114.36 Da
• Isoelectric Point: 6.9781

>T30204 WP_022775091.1 NC_022576:c2266101-2265700 [Candidatus Symbiobacter mobilis CR]
MIWMLDTNICSYVMRTLPVTVQRRFESIGPEYLAISAVVLAELRYGAARHPTRSTLYFAEIDNFTSRLDVLAWTSHAADH
YARLRATLERRGQPIGNMDMLIAAHALAENAVLVTHNVREFERVPGLTVENWA

Nucleotide

• Length: 402 bp

>T30204 NC_022576:c2266101-2265700 [Candidatus Symbiobacter mobilis CR]
ATGATTTGGATGCTTGACACCAATATCTGCAGCTATGTGATGCGTACGCTGCCAGTGACGGTACAGCGCCGATTCGAAAG
CATCGGCCCGGAGTACCTTGCCATTTCTGCCGTCGTGCTTGCCGAATTGCGTTACGGCGCAGCCCGCCACCCCACTCGCT
CGACTTTGTATTTTGCGGAAATCGACAACTTCACCAGTCGACTGGATGTTTTGGCCTGGACATCGCATGCTGCGGACCAC
TACGCTCGCTTGCGCGCCACCTTGGAACGACGGGGACAACCTATCGGCAATATGGACATGCTCATTGCTGCCCATGCATT
GGCCGAAAACGCCGTGCTGGTCACCCACAATGTTCGTGAATTCGAGCGGGTGCCAGGGTTGACAGTAGAGAACTGGGCGT
AA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9813.07 Da
• Isoelectric Point: 5.8432

>AT30204 WP_022775095.1 NC_022576:c2266336-2266085 [Candidatus Symbiobacter mobilis CR]
MQQTAKLFRNGRSQAVRLPEEFRFEGQEVYIEKQGNAVILKPKPKPKTWDDFFARTPAMPTDFLLDRQDEPPQDRQWPHD
LDA

Nucleotide

• Length: 252 bp

>AT30204 NC_022576:c2266336-2266085 [Candidatus Symbiobacter mobilis CR]
ATGCAACAAACCGCCAAACTATTCCGTAACGGACGTAGTCAAGCCGTGCGCCTGCCCGAGGAATTCCGGTTCGAGGGCCA
AGAGGTGTACATCGAAAAACAGGGGAATGCCGTCATCCTCAAACCCAAACCCAAACCCAAGACTTGGGACGATTTTTTTG
CACGCACGCCAGCAATGCCGACGGATTTCCTGCTGGATCGGCAAGACGAACCGCCTCAGGATCGGCAATGGCCCCATGAT
TTGGATGCTTGA

Structures

Toxin

• AlphaFold DB: U5ND13

Antitoxin

• AlphaFold DB: U5ND78