Detailed information of TA system TA030202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-relB/BrnT-dnstrm_HI1420
• Location: 2153666..2154322
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: CENROD_RS08815
• Protein ID: WP_041193453.1
• Coordinates: 2153666..2153938 (-)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U5N9N9
• Locus tag: CENROD_RS08820
• Protein ID: WP_022774767.1
• Coordinates: 2154035..2154322 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS13150	2149248..2150144	-	897	Protein_1756	hypothetical protein	-
CENROD_RS08805	2150153..2152441	-	2289	Protein_1757	ATP-dependent exonuclease SbcCD, C subunit-like protein	-
CENROD_RS08810	2152434..2153114	-	681	WP_022774759.1	DUF4194 domain-containing protein	-
CENROD_RS13155	2153449..2153679	-	231	WP_022774763.1	hypothetical protein	-
CENROD_RS08815	2153666..2153938	-	273	WP_041193453.1	BrnT family toxin	Toxin
CENROD_RS08820	2154035..2154322	-	288	WP_022774767.1	putative addiction module antidote protein	Antitoxin
CENROD_RS13160	2154315..2154626	-	312	WP_022774771.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CENROD_RS08825	2154930..2155595	+	666	WP_022774775.1	retron-type reverse transcriptase	-
CENROD_RS08830	2155592..2155930	-	339	WP_202961146.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CENROD_RS13165	2155885..2156112	-	228	WP_022774782.1	addiction module protein	-
CENROD_RS08835	2156398..2157708	+	1311	WP_022774790.1	group II intron reverse transcriptase/maturase	-
CENROD_RS08840	2157755..2158390	-	636	WP_022774794.1	hypothetical protein	-
CENROD_RS13625	2158397..2158741	-	345	WP_151194609.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-83)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10547.65 Da
• Isoelectric Point: 5.5693

>T30202 WP_041193453.1 NC_022576:c2153938-2153666 [Candidatus Symbiobacter mobilis CR]
MTYEWDEHKATANLSKHGVSFEEAKTVFDDPLYVDFYDPDHSYGEHRFILLGQSKQGRLLFVSYMERNGSIRLISAREAT
PSERRAYEQN

Nucleotide

• Length: 273 bp

>T30202 NC_022576:c2153938-2153666 [Candidatus Symbiobacter mobilis CR]
ATGACATACGAATGGGATGAACATAAAGCGACAGCGAACTTGTCGAAGCATGGTGTTTCTTTTGAGGAAGCCAAAACAGT
TTTCGATGATCCGTTGTATGTGGATTTCTACGATCCTGACCATTCCTACGGGGAACACCGTTTTATCTTGCTGGGTCAAT
CGAAACAAGGGCGATTATTATTCGTCTCGTACATGGAGCGTAACGGCTCCATCCGTTTAATCAGCGCAAGGGAAGCAACG
CCATCGGAGCGTAGAGCATATGAACAAAATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10343.83 Da
• Isoelectric Point: 5.0745

>AT30202 WP_022774767.1 NC_022576:c2154322-2154035 [Candidatus Symbiobacter mobilis CR]
MVKFSPFDAADYLDDEETIAEYITAALEDSNPDVFLTAVRDVARARGMSQLAKDAGLGRESLYKALMPGAKPRYDTMLKL
LHALGVKLSASPVHS

Nucleotide

• Length: 288 bp

>AT30202 NC_022576:c2154322-2154035 [Candidatus Symbiobacter mobilis CR]
ATGGTTAAATTTTCCCCATTCGATGCCGCCGACTATCTCGACGATGAAGAAACCATCGCGGAATACATTACTGCTGCGCT
TGAAGATTCCAACCCAGATGTGTTTCTAACCGCAGTGCGAGATGTGGCTCGCGCTCGTGGTATGTCACAACTCGCCAAAG
ATGCAGGCCTTGGGCGCGAAAGCCTCTACAAAGCGCTTATGCCCGGCGCAAAGCCGCGCTATGACACAATGCTCAAGTTA
CTTCATGCTCTCGGTGTAAAGCTCTCTGCTTCACCCGTCCATTCATGA

Structures

Antitoxin

• AlphaFold DB: U5N9N9