Detailed information of TA system TA030201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1800037..1800578
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N834
• Locus tag: CENROD_RS07375
• Protein ID: WP_022773672.1
• Coordinates: 1800255..1800578 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CENROD_RS07370
• Protein ID: WP_041194305.1
• Coordinates: 1800037..1800258 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS07350	1795129..1796475	-	1347	WP_022773657.1	hypothetical protein	-
CENROD_RS07355	1796485..1798197	-	1713	WP_022773661.1	dihydroxy-acid dehydratase	-
CENROD_RS07360	1798397..1799212	+	816	WP_022773664.1	prolipoprotein diacylglyceryl transferase	-
CENROD_RS07365	1799134..1799934	+	801	WP_081699843.1	tRNA (guanosine(46)-N7)-methyltransferase TrmB	-
CENROD_RS07370	1800037..1800258	+	222	WP_041194305.1	antitoxin MazE family protein	Antitoxin
CENROD_RS07375	1800255..1800578	+	324	WP_022773672.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CENROD_RS07380	1800555..1801577	-	1023	WP_081699934.1	16S rRNA (cytidine(1402)-2'-O)-methyltransferase	-
CENROD_RS07385	1801612..1802511	+	900	WP_022773679.1	protease HtpX	-
CENROD_RS07390	1802653..1803504	-	852	WP_202961142.1	cation diffusion facilitator family transporter	-
CENROD_RS07395	1803660..1804172	-	513	WP_022773686.1	pilin	-
CENROD_RS13580	1804301..1804573	-	273	WP_022773690.1	hypothetical protein	-
CENROD_RS07400	1804608..1804796	-	189	WP_022773693.1	DUF1778 domain-containing protein	-
CENROD_RS07405	1805074..1805469	+	396	WP_022773700.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11761.74 Da
• Isoelectric Point: 7.9847

>T30201 WP_022773672.1 NC_022576:1800255-1800578 [Candidatus Symbiobacter mobilis CR]
MTQRGDLVMVSLQGNYGKPRPALVIQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRTLSQVMVDKLSTLPRTK
ISEPFGRLDEDRMKTVDRALLLVVGVI

Nucleotide

• Length: 324 bp

>T30201 NC_022576:1800255-1800578 [Candidatus Symbiobacter mobilis CR]
ATGACGCAACGAGGCGACTTGGTGATGGTTTCTCTGCAAGGCAACTATGGCAAACCGCGCCCAGCCTTGGTCATCCAATC
AGACCTCTTGACGGATTTGGAAAGCGTTGTGCTGTGCCCGGTCACAAGCGATCTGCGCAATGCCGCATTTCGCGTGACCG
TGGAACCGAATCCCGCCAATGGGTTGCGCACGCTTTCGCAAGTCATGGTAGACAAACTTTCGACGCTGCCGCGTACCAAA
ATCAGCGAACCGTTTGGCCGTCTTGATGAAGACAGGATGAAGACGGTAGACCGGGCCTTGCTACTGGTCGTTGGCGTGAT
CTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8351.34 Da
• Isoelectric Point: 6.2142

>AT30201 WP_041194305.1 NC_022576:1800037-1800258 [Candidatus Symbiobacter mobilis CR]
MASIHESTSKVARHRERMRAAGLRPVQFWVPDTRSPEFMAQVRQQCQSLQGDPAEADVLRFTEEAATHVEGWE

Nucleotide

• Length: 222 bp

>AT30201 NC_022576:1800037-1800258 [Candidatus Symbiobacter mobilis CR]
ATGGCCTCAATCCACGAATCGACCAGCAAGGTAGCGCGTCACCGCGAGCGGATGCGTGCTGCGGGGCTGCGGCCTGTGCA
GTTTTGGGTGCCGGACACCCGCTCGCCGGAGTTCATGGCGCAGGTGCGCCAGCAGTGCCAAAGTCTCCAGGGCGACCCGG
CAGAAGCCGACGTTTTGCGCTTCACCGAAGAGGCCGCAACCCATGTTGAGGGTTGGGAATGA

Structures

Toxin

• AlphaFold DB: U5N834