Detailed information of TA system TA030199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1484604..1485187
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N812
• Locus tag: CENROD_RS06115
• Protein ID: WP_022772727.1
• Coordinates: 1484846..1485187 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U5NB94
• Locus tag: CENROD_RS06110
• Protein ID: WP_022772723.1
• Coordinates: 1484604..1484846 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS06095	1480010..1481605	+	1596	WP_022772712.1	AAA-like domain-containing protein	-
CENROD_RS06100	1481607..1482365	+	759	WP_022772716.1	Fic family protein	-
CENROD_RS12465	1482492..1484324	-	1833	WP_022772720.1	TerD family protein	-
CENROD_RS06110	1484604..1484846	+	243	WP_022772723.1	signal transduction protein	Antitoxin
CENROD_RS06115	1484846..1485187	+	342	WP_022772727.1	endoribonuclease MazF	Toxin
CENROD_RS06120	1485246..1485395	+	150	WP_022772731.1	putative addiction module antidote protein	-
CENROD_RS06125	1485516..1486082	+	567	WP_187292350.1	aminodeoxychorismate/anthranilate synthase component II	-
CENROD_RS06130	1486153..1486920	-	768	WP_022772738.1	WecB/TagA/CpsF family glycosyltransferase	-
CENROD_RS06135	1487142..1488197	-	1056	WP_187292341.1	glycosyltransferase family 4 protein	-
CENROD_RS13065	1488303..1488905	-	603	WP_151194596.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• Antitoxin PemI-like (1-80)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12340.45 Da
• Isoelectric Point: 9.2150

>T30199 WP_022772727.1 NC_022576:1484846-1485187 [Candidatus Symbiobacter mobilis CR]
MPRAYVPDTGEVVWLEFDPQAGHEQAGHRPALVISPASYNGKTGLMVCCPMSTKIKGHPFEVVTKVDGVDCAVLSDQVKS
LDWKVRQAKRKSVVSADVILHVKAKMKALLQIS

Nucleotide

• Length: 342 bp

>T30199 NC_022576:1484846-1485187 [Candidatus Symbiobacter mobilis CR]
ATGCCACGTGCTTATGTCCCCGATACAGGCGAAGTGGTTTGGCTGGAATTTGATCCACAGGCTGGGCATGAACAGGCCGG
CCACCGGCCTGCGTTGGTCATCAGCCCAGCAAGCTACAACGGGAAAACCGGACTGATGGTGTGTTGCCCAATGTCAACCA
AGATCAAAGGGCACCCCTTCGAAGTCGTCACCAAGGTCGATGGCGTGGATTGCGCGGTGCTTTCAGATCAAGTGAAATCC
CTCGACTGGAAGGTCCGGCAGGCCAAAAGAAAATCTGTCGTCAGCGCCGATGTGATACTGCATGTCAAAGCAAAGATGAA
GGCCTTGTTGCAGATTTCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8761.95 Da
• Isoelectric Point: 4.7023

>AT30199 WP_022772723.1 NC_022576:1484604-1484846 [Candidatus Symbiobacter mobilis CR]
MEAVIRKWGNSPALRLPTTVLREAGYQLEQKVDLIVSRGRIIIQPSEKVEYDLDALISGINATNAHDEVSFGSPVGNEAL

Nucleotide

• Length: 243 bp

>AT30199 NC_022576:1484604-1484846 [Candidatus Symbiobacter mobilis CR]
ATGGAAGCAGTCATTCGCAAATGGGGTAACAGCCCCGCCCTGCGCCTGCCAACCACCGTTTTGAGGGAGGCGGGTTATCA
ATTGGAGCAGAAGGTCGATTTGATCGTCTCGCGTGGTCGCATCATCATTCAGCCATCTGAAAAAGTTGAATATGACCTTG
ATGCACTCATCAGTGGCATCAATGCGACCAATGCTCACGATGAAGTCAGCTTTGGTTCGCCCGTTGGCAATGAGGCACTC
TGA

Structures

Toxin

• AlphaFold DB: U5N812

Antitoxin

• AlphaFold DB: U5NB94