Detailed information of TA system TA030194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 552627..554137
• Replicon: chromosome
• Accession: NC_022576
• Organism: Candidatus Symbiobacter mobilis CR

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: CENROD_RS02260
• Protein ID: WP_081699793.1
• Coordinates: 553223..554137 (+)
• Length: 305 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: U5N531
• Locus tag: CENROD_RS02255
• Protein ID: WP_022771452.1
• Coordinates: 552627..553226 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CENROD_RS13450	547964..548545	-	582	WP_202961173.1	acyltransferase	-
CENROD_RS12955	548651..549973	-	1323	WP_187292320.1	lipopolysaccharide biosynthesis protein	-
CENROD_RS02240	549954..550961	-	1008	WP_022771448.1	NAD-dependent epimerase	-
CENROD_RS02245	551470..551853	+	384	WP_022771450.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CENROD_RS02250	551858..552208	+	351	WP_022771451.1	XRE family transcriptional regulator	-
CENROD_RS02255	552627..553226	+	600	WP_022771452.1	AbiEi antitoxin N-terminal domain-containing protein	Antitoxin
CENROD_RS02260	553223..554137	+	915	WP_081699793.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
CENROD_RS02265	554175..554789	-	615	WP_022771454.1	hypothetical protein	-
CENROD_RS02270	555128..555511	-	384	WP_022771456.1	AAA family ATPase	-
CENROD_RS13810	555616..555816	-	201	WP_022771457.1	nucleoside-diphosphate sugar epimerase	-
CENROD_RS13815	555859..556044	-	186	WP_022771458.1	NAD-dependent epimerase/dehydratase family protein	-
CENROD_RS02280	556465..557472	-	1008	WP_022771460.1	Rpn family recombination-promoting nuclease/putative transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (36-261)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-51)

Sequences

Toxin

Protein

• Length: 305 a.a.
• Molecular weight: 34363.29 Da
• Isoelectric Point: 6.6116

>T30194 WP_081699793.1 NC_022576:553223-554137 [Candidatus Symbiobacter mobilis CR]
MNQRNTAASVRARLLTRARETRQDFNLVLTRYTLERLLYRLSVSHHADQFLLKGALLFDLWFDIPHRPTRDADLLGFGSA
ELPHLEAVFKDICAMETDDGVTFRPDTVHAAEIRKQANYAGVRVTLLGLIDGARCPIQVDIGFGDAVTPGPEDVQYPVML
PEFAAPKLRVYPRYTVVAEKLEALVSLGIANSRMKDYFDLWILSRYTEFDGDTLRRAIRATFDRRKTMLPLDTPFGLTDA
FAQDAQKQRQWQAFLGKNRLEALHLEDIVASLRDFLLPIIAAANVDADYSQHWPSGGLWSSVVG

Nucleotide

• Length: 915 bp

>T30194 NC_022576:553223-554137 [Candidatus Symbiobacter mobilis CR]
ATGAACCAGCGCAACACGGCTGCTTCGGTGCGGGCGCGCCTGCTCACCCGCGCCCGCGAGACCCGGCAAGACTTCAATCT
GGTCTTGACCCGCTACACCCTTGAACGCCTGCTGTACCGGCTCAGTGTCTCGCATCATGCCGACCAGTTCCTGCTGAAAG
GCGCGCTGTTGTTCGACTTGTGGTTCGATATTCCGCATCGACCGACGCGGGACGCGGACTTGCTGGGTTTTGGTTCGGCC
GAGTTGCCGCACCTCGAGGCGGTCTTCAAGGACATCTGCGCGATGGAGACTGATGACGGGGTGACGTTTCGACCGGACAC
TGTCCATGCGGCGGAGATTCGCAAGCAGGCCAACTATGCTGGCGTGCGCGTGACCTTACTTGGACTGATCGATGGAGCAC
GCTGCCCGATTCAGGTCGACATCGGCTTCGGCGATGCCGTCACACCAGGGCCGGAAGATGTCCAGTACCCCGTGATGCTA
CCGGAGTTCGCGGCGCCGAAACTGCGCGTCTATCCGCGCTACACCGTGGTGGCAGAAAAGCTGGAGGCGTTGGTCTCGCT
CGGCATCGCCAACAGCCGAATGAAGGATTACTTCGACCTTTGGATCTTGTCGCGGTACACCGAATTTGACGGCGACACCC
TGCGACGTGCGATTCGGGCAACCTTCGATCGACGCAAGACGATGTTGCCCCTGGACACCCCCTTTGGCCTGACGGATGCT
TTTGCGCAAGATGCGCAAAAGCAGAGGCAGTGGCAGGCGTTTTTGGGAAAGAACAGACTCGAAGCGCTGCACCTGGAGGA
CATCGTGGCATCACTTCGAGATTTTCTGCTCCCAATCATCGCGGCAGCGAACGTTGATGCCGACTACTCGCAACACTGGC
CATCTGGCGGACTCTGGTCATCAGTGGTGGGGTGA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22155.67 Da
• Isoelectric Point: 9.2871

>AT30194 WP_022771452.1 NC_022576:552627-553226 [Candidatus Symbiobacter mobilis CR]
METSQQTILNLAAQRGLIRPCDLDARGLPSVALTRLVRQGLLTRVGRGLYACPDRSISEHGSLAEVARKHPQAIVCLLSA
LRVNDLTTQSPFEVWVAIPNKARAPKMDYPPLRIVRFSGAALTDGIDEHQIDGATVRVTNVARTVADCFKFRNKIGIDVG
MEALQEAWHTKRVSMDELWRYATLCRVANVMRPYMESLS

Nucleotide

• Length: 600 bp

>AT30194 NC_022576:552627-553226 [Candidatus Symbiobacter mobilis CR]
ATGGAAACTTCGCAGCAGACCATTCTGAATCTCGCCGCCCAGCGCGGTCTCATCCGCCCGTGCGATCTCGATGCGCGGGG
TCTGCCCAGTGTCGCCCTCACGCGGCTGGTTCGGCAGGGGCTGCTCACCCGTGTGGGTCGCGGTCTGTATGCCTGTCCTG
ATCGCAGCATATCTGAGCACGGCTCCCTGGCGGAGGTGGCACGCAAACACCCGCAAGCCATCGTCTGCCTGCTGTCGGCT
CTACGGGTGAACGATCTCACCACGCAGTCACCGTTCGAGGTCTGGGTCGCCATTCCCAACAAAGCGCGTGCGCCGAAGAT
GGATTACCCACCGCTGCGCATCGTTCGCTTCTCAGGTGCCGCGCTGACGGATGGAATTGACGAGCACCAGATCGATGGCG
CGACTGTGCGTGTAACCAACGTCGCACGTACCGTGGCCGATTGCTTCAAGTTTCGCAACAAGATCGGCATAGATGTTGGG
ATGGAAGCGCTACAAGAGGCATGGCATACCAAACGCGTGAGCATGGACGAACTCTGGCGCTATGCCACGCTGTGTCGCGT
GGCCAATGTAATGCGCCCTTACATGGAAAGCCTGTCATGA

Structures

Antitoxin

• AlphaFold DB: U5N531