Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2582984..2583574 Replicon chromosome
Accession NC_022566
Organism Klebsiella pneumoniae CG43

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag D364_RS12775 Protein ID WP_022615588.1
Coordinates 2582984..2583316 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag D364_RS12780 Protein ID WP_000288812.1
Coordinates 2583317..2583574 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D364_RS12745 2579031..2579276 + 246 WP_004141189.1 signal transduction protein PmrD -
D364_RS12750 2579406..2580860 + 1455 WP_004148975.1 AMP nucleosidase -
D364_RS12760 2581226..2582662 - 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
D364_RS12775 2582984..2583316 - 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D364_RS12780 2583317..2583574 - 258 WP_000288812.1 antitoxin Antitoxin
D364_RS12785 2583909..2584115 - 207 WP_022615589.1 helix-turn-helix domain-containing protein -
D364_RS12790 2584112..2584573 - 462 WP_004213450.1 hypothetical protein -
D364_RS26170 2584856..2585412 - 557 Protein_2470 hypothetical protein -
D364_RS26175 2585491..2587239 - 1749 Protein_2471 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T30184 WP_022615588.1 NC_022566:c2583316-2582984 [Klebsiella pneumoniae CG43]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T30184 NC_022566:c2583316-2582984 [Klebsiella pneumoniae CG43]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT30184 WP_000288812.1 NC_022566:c2583574-2583317 [Klebsiella pneumoniae CG43]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT30184 NC_022566:c2583574-2583317 [Klebsiella pneumoniae CG43]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References