Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 478504..479123 Replicon chromosome
Accession NC_022566
Organism Klebsiella pneumoniae CG43

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag D364_RS02295 Protein ID WP_002892050.1
Coordinates 478504..478722 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag D364_RS02300 Protein ID WP_002892066.1
Coordinates 478749..479123 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D364_RS02260 474551..474814 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
D364_RS02265 474814..474954 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
D364_RS02270 474951..475649 - 699 WP_004177238.1 GNAT family N-acetyltransferase -
D364_RS02275 475750..477201 + 1452 WP_004213726.1 PLP-dependent aminotransferase family protein -
D364_RS02280 477176..477646 - 471 WP_002892026.1 YlaC family protein -
D364_RS27275 477667..477807 + 141 WP_004147370.1 hypothetical protein -
D364_RS02290 477779..478345 - 567 WP_002892030.1 maltose O-acetyltransferase -
D364_RS02295 478504..478722 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
D364_RS02300 478749..479123 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
D364_RS02305 479609..482755 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
D364_RS02310 482778..483971 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T30174 WP_002892050.1 NC_022566:c478722-478504 [Klebsiella pneumoniae CG43]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T30174 NC_022566:c478722-478504 [Klebsiella pneumoniae CG43]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT30174 WP_002892066.1 NC_022566:c479123-478749 [Klebsiella pneumoniae CG43]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT30174 NC_022566:c479123-478749 [Klebsiella pneumoniae CG43]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References