Detailed information of TA system TA030159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 561752..562350
• Replicon: plasmid IRBL74_p
• Accession: NC_022536
• Organism: Rhizobium sp. IRBG74

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U4QIX0
• Locus tag: BN877_RS25740
• Protein ID: WP_022557667.1
• Coordinates: 561752..562045 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U4Q8S4
• Locus tag: BN877_RS25745
• Protein ID: WP_022557668.1
• Coordinates: 562042..562350 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN877_RS25710	556972..557172	+	201	WP_006699143.1	type II toxin-antitoxin system VapB family antitoxin	-
BN877_RS25720	557682..557993	-	312	WP_022557664.1	type II toxin-antitoxin system RelE/ParE family toxin	-
BN877_RS25725	557990..558241	-	252	WP_022557665.1	Antitoxin ParD	-
BN877_RS25730	558690..558989	+	300	WP_034499457.1	hypothetical protein	-
BN877_RS25735	559243..561177	-	1935	WP_022557666.1	HAMP domain-containing protein	-
BN877_RS25740	561752..562045	-	294	WP_022557667.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
BN877_RS25745	562042..562350	-	309	WP_022557668.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
BN877_RS25750	562546..563775	-	1230	WP_034499453.1	HipA domain-containing protein	-
BN877_RS25755	563768..564358	-	591	WP_022557670.1	helix-turn-helix transcriptional regulator	-
BN877_RS25760	564593..564979	+	387	Protein_554	IS6 family transposase	-
BN877_RS25765	565352..566383	-	1032	WP_081859572.1	polyamine ABC transporter substrate-binding protein	-
BN877_RS25770	566468..567181	+	714	WP_022557673.1	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / gmd	1..584965	584965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10505.03 Da
• Isoelectric Point: 5.7996

>T30159 WP_022557667.1 NC_022536:c562045-561752 [Rhizobium pusense]
VKLTWSAFALSDRDAIFTYVEADNPAAAVLIDERIAAAARRLLDFPASGRVGRIAGTRELVINGTPYIAAYAVAETAVRI
LRVLHGAQEWPENLPLS

Nucleotide

• Length: 294 bp

>T30159 NC_022536:c562045-561752 [Rhizobium pusense]
GTGAAGCTTACTTGGTCTGCTTTTGCGTTGTCGGATCGTGACGCCATCTTCACATACGTCGAGGCAGATAATCCCGCCGC
TGCCGTGTTGATCGATGAACGGATAGCCGCTGCCGCCCGTCGCTTGCTCGATTTTCCTGCCAGTGGGCGCGTGGGTCGAA
TTGCCGGGACACGCGAACTCGTCATCAATGGCACGCCTTATATCGCCGCCTACGCCGTTGCCGAGACGGCAGTTCGAATT
CTTCGCGTACTCCATGGCGCGCAAGAATGGCCAGAAAATCTGCCGCTAAGCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11098.46 Da
• Isoelectric Point: 5.1704

>AT30159 WP_022557668.1 NC_022536:c562350-562042 [Rhizobium pusense]
MAANALVQTRIDSEVRDRASAVLESMGLTVSDAVRIMLTRTANEGALPLELLSGSEAHDAWFRTKVHEALNDTRPDLSDD
EAEAHFVKRRAAARLKAGVPES

Nucleotide

• Length: 309 bp

>AT30159 NC_022536:c562350-562042 [Rhizobium pusense]
ATGGCCGCAAACGCTCTCGTACAAACACGTATTGATTCCGAAGTCAGGGATCGTGCTTCTGCCGTCCTTGAAAGCATGGG
CCTCACGGTATCTGATGCGGTTCGTATCATGCTCACCCGGACCGCTAACGAGGGCGCTCTGCCTCTTGAACTTTTATCCG
GCAGTGAGGCGCATGACGCTTGGTTTCGGACCAAGGTGCATGAAGCGTTAAACGATACTCGACCGGATTTATCGGACGAC
GAGGCCGAGGCGCACTTTGTCAAACGTCGAGCGGCAGCCCGCCTTAAAGCGGGTGTGCCAGAATCGTGA

Structures

Toxin

• AlphaFold DB: U4QIX0

Antitoxin

• AlphaFold DB: U4Q8S4