Detailed information of TA system TA030158

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 117326..117996
• Replicon: plasmid IRBL74_p
• Accession: NC_022536
• Organism: Rhizobium sp. IRBG74

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U4QHU8
• Locus tag: BN877_RS23575
• Protein ID: WP_022557187.1
• Coordinates: 117326..117745 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4Q725
• Locus tag: BN877_RS26700
• Protein ID: WP_006699039.1
• Coordinates: 117742..117996 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN877_RS23550	112434..113624	+	1191	WP_022557180.1	hypothetical protein	-
BN877_RS23555	113731..113985	+	255	WP_034499700.1	hypothetical protein	-
BN877_RS23560	114113..114565	+	453	WP_022557183.1	hypothetical protein	-
BN877_RS23565	114578..115615	+	1038	WP_022557185.1	toprim domain-containing protein	-
BN877_RS23570	115945..116886	+	942	WP_022557186.1	DUF2493 domain-containing protein	-
BN877_RS23575	117326..117745	-	420	WP_022557187.1	type II toxin-antitoxin system VapC family toxin	Toxin
BN877_RS26700	117742..117996	-	255	WP_006699039.1	hypothetical protein	Antitoxin
BN877_RS27250	118152..118349	-	198	WP_144115359.1	hypothetical protein	-
BN877_RS23585	118449..118955	-	507	WP_006699037.1	RES domain-containing protein	-
BN877_RS23590	118952..119437	-	486	WP_022557190.1	DUF2384 domain-containing protein	-
BN877_RS23600	119843..120166	+	324	WP_006699035.1	DUF736 family protein	-
BN877_RS27610	120269..120499	-	231	WP_048903009.1	hypothetical protein	-
BN877_RS23610	120622..120900	-	279	WP_022557192.1	HU family DNA-binding protein	-
BN877_RS23615	121247..121456	+	210	WP_045535234.1	hypothetical protein	-
BN877_RS23620	121647..121922	-	276	WP_022557194.1	WGR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / gmd	1..584965	584965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15054.14 Da
• Isoelectric Point: 4.8728

>T30158 WP_022557187.1 NC_022536:c117745-117326 [Rhizobium pusense]
MILLDTNVVSETMKPFPSQMVQRWLDEQAAETLHLSSITIAEAMFGIRTMPDGQRKQRLAEALDVWIGLFEGRILPFDLD
AAKHYANLGSSARASGKGFPTTDGYIAAIAASKGFAVATRDASAFHAAGLEVIDPWTAE

Nucleotide

• Length: 420 bp

>T30158 NC_022536:c117745-117326 [Rhizobium pusense]
ATGATTCTGCTAGACACCAATGTCGTCTCGGAGACGATGAAGCCTTTTCCGAGCCAAATGGTACAGCGCTGGCTCGACGA
GCAAGCTGCCGAGACCCTCCATCTTTCGAGTATCACGATCGCGGAGGCCATGTTCGGCATCCGCACCATGCCCGATGGTC
AGCGCAAGCAGAGGCTCGCGGAGGCTCTCGATGTCTGGATCGGCCTGTTCGAGGGACGAATTTTGCCCTTCGATCTCGAT
GCCGCCAAACACTATGCCAATCTCGGATCTTCGGCCCGTGCATCGGGGAAGGGATTCCCGACAACCGACGGCTACATTGC
GGCCATCGCTGCATCCAAGGGCTTCGCAGTCGCGACCCGTGACGCCAGCGCGTTTCATGCCGCCGGTCTTGAAGTCATTG
ATCCCTGGACGGCCGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9406.71 Da
• Isoelectric Point: 6.7270

>AT30158 WP_006699039.1 NC_022536:c117996-117742 [Rhizobium pusense]
MPAVTIRNIPEETHRAIKIRAAQHGRSAEAEMRDILEMAVRPATRLRLGTALAEASRRIGLTNEDVDALEQTFDRTPAHP
MSFE

Nucleotide

• Length: 255 bp

>AT30158 NC_022536:c117996-117742 [Rhizobium pusense]
ATGCCCGCCGTAACAATCCGAAACATACCCGAGGAAACGCATCGCGCCATCAAGATCCGTGCGGCACAGCATGGCCGCAG
CGCCGAGGCCGAAATGCGCGACATTCTGGAAATGGCCGTTCGGCCCGCAACCCGTCTCCGTCTCGGCACGGCACTCGCCG
AGGCCAGTCGTCGCATCGGCCTCACCAACGAGGACGTCGATGCCCTTGAGCAGACCTTTGATCGCACGCCAGCCCATCCC
ATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: U4QHU8

Antitoxin

• AlphaFold DB: U4Q725