30156

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 653136..653622 Replicon chromosome
Accession NC_022535
Organism Rhizobium sp. IRBG74

Toxin (Protein)


Gene name pasB Uniprot ID U4PR09
Locus tag BN877_RS03105 Protein ID WP_022555687.1
Coordinates 653136..653405 (-) Length 90 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID A0A1S7Q717
Locus tag BN877_RS03110 Protein ID WP_004440186.1
Coordinates 653389..653622 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN877_RS03070 648235..648864 + 630 WP_004440176.1 DNA-3-methyladenine glycosylase I -
BN877_RS03075 648861..649304 + 444 WP_045532658.1 hypothetical protein -
BN877_RS03080 649319..650362 - 1044 WP_022555682.1 YeiH family protein -
BN877_RS03085 650444..651361 + 918 WP_004440182.1 LysR family transcriptional regulator -
BN877_RS03090 651508..651882 - 375 WP_022555684.1 DUF305 domain-containing protein -
BN877_RS03095 651940..652299 - 360 WP_048902656.1 hypothetical protein -
BN877_RS03100 652365..653129 - 765 WP_022555686.1 hypothetical protein -
BN877_RS03105 653136..653405 - 270 WP_022555687.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BN877_RS03110 653389..653622 - 234 WP_004440186.1 ribbon-helix-helix protein, CopG family Antitoxin
BN877_RS03115 653857..655380 + 1524 WP_022555688.1 histidine--tRNA ligase -
BN877_RS03120 655385..655753 + 369 WP_006699502.1 VOC family protein -
BN877_RS03125 655770..656894 + 1125 WP_004440189.1 ATP phosphoribosyltransferase regulatory subunit -
BN877_RS03130 656891..657583 + 693 WP_022555689.1 ATP phosphoribosyltransferase -
BN877_RS03135 657646..658092 - 447 WP_004440191.1 DoxX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-88)

Antitoxin

Protein of unknown function DUF6290

(6-73)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10478.05 Da        Isoelectric Point: 9.5233

>T30156 WP_022555687.1 NC_022535:c653405-653136 [Rhizobium pusense]
MIWKIEFQHRAVKQLEKLAKQDANRIVSFLADGVVQNENPRQTGAALQGSELGNYWRYRVGDYRIICDIQDHKLIVLVVE
IGHRREIYR

Download         Length: 270 bp

>T30156 NC_022535:c653405-653136 [Rhizobium pusense]
TTGATCTGGAAAATTGAGTTTCAGCACCGCGCCGTCAAGCAGCTTGAAAAGCTGGCGAAGCAAGATGCGAACCGCATCGT
TTCGTTTCTGGCAGACGGGGTGGTGCAAAATGAAAATCCCCGCCAGACTGGTGCCGCCCTGCAAGGCTCCGAACTCGGCA
ATTATTGGCGCTACCGGGTGGGCGATTATCGCATCATCTGCGATATACAGGATCACAAGCTAATCGTGCTGGTGGTCGAA
ATCGGCCATCGCCGCGAAATTTACCGTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 9225.31 Da        Isoelectric Point: 4.7359

>AT30156 WP_004440186.1 NC_022535:c653622-653389 [Rhizobium pusense]
MSKQTAIRLPDETYERLKALSERTGRTSAYYIREAIEKHIEDMEDLYLAEEATRRRIANNERTYTLEEVMRNLDLEN

Download         Length: 234 bp

>AT30156 NC_022535:c653622-653389 [Rhizobium pusense]
ATGAGCAAACAGACGGCCATCCGCCTCCCGGATGAGACCTATGAGCGGCTGAAAGCGCTTTCCGAGCGCACCGGACGCAC
ATCTGCCTATTACATTCGCGAGGCGATCGAGAAGCATATCGAAGATATGGAAGATCTTTATCTCGCCGAAGAGGCGACGC
GGCGACGGATCGCAAACAATGAGCGCACCTATACTCTGGAAGAAGTGATGCGCAATCTTGATCTGGAAAATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U4PR09

Antitoxin

Source ID Structure
AlphaFold DB A0A1S7Q717

References