Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 488612..489249 | Replicon | |
| Accession | NC_022530 | ||
| Organism | Bacillus amyloliquefaciens subsp. plantarum NAU-B3 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | BAPNAU_RS02465 | Protein ID | WP_003156187.1 |
| Coordinates | 488899..489249 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | BAPNAU_RS02460 | Protein ID | WP_003156188.1 |
| Coordinates | 488612..488893 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BAPNAU_RS02440 | 484977..485576 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| BAPNAU_RS02445 | 485669..486034 | + | 366 | WP_022552632.1 | holo-ACP synthase | - |
| BAPNAU_RS02450 | 486199..487206 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| BAPNAU_RS02455 | 487323..488492 | + | 1170 | WP_022552633.1 | alanine racemase | - |
| BAPNAU_RS02460 | 488612..488893 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| BAPNAU_RS02465 | 488899..489249 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| BAPNAU_RS02470 | 489367..490188 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| BAPNAU_RS02475 | 490193..490558 | + | 366 | WP_007609584.1 | RsbT antagonist protein RsbS | - |
| BAPNAU_RS02480 | 490561..490962 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| BAPNAU_RS02485 | 490974..491981 | + | 1008 | WP_007609589.1 | PP2C family protein-serine/threonine phosphatase | - |
| BAPNAU_RS02490 | 492045..492374 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| BAPNAU_RS02495 | 492371..492853 | + | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| BAPNAU_RS02500 | 492819..493607 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| BAPNAU_RS02505 | 493607..494209 | + | 603 | WP_007410234.1 | SpoIIE family protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T30150 WP_003156187.1 NC_022530:488899-489249 [Bacillus velezensis NAU-B3]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T30150 NC_022530:488899-489249 [Bacillus velezensis NAU-B3]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT30150 WP_003156188.1 NC_022530:488612-488893 [Bacillus velezensis NAU-B3]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT30150 NC_022530:488612-488893 [Bacillus velezensis NAU-B3]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|