Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 257658..258294 Replicon chromosome
Accession NC_022524
Organism Bacillus infantis NRRL B-14911

Toxin (Protein)


Gene name mazF Uniprot ID U5L4U1
Locus tag N288_RS01460 Protein ID WP_009795787.1
Coordinates 257944..258294 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q2B506
Locus tag N288_RS01455 Protein ID WP_009795786.1
Coordinates 257658..257939 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N288_RS01435 253372..253962 - 591 WP_009795780.1 rhomboid family intramembrane serine protease -
N288_RS01440 254092..254460 + 369 WP_035403472.1 holo-ACP synthase -
N288_RS01445 254667..255683 + 1017 WP_022543252.1 outer membrane lipoprotein carrier protein LolA -
N288_RS01450 256275..257447 + 1173 WP_035403475.1 alanine racemase -
N288_RS01455 257658..257939 + 282 WP_009795786.1 hypothetical protein Antitoxin
N288_RS01460 257944..258294 + 351 WP_009795787.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N288_RS01465 258702..259535 + 834 WP_009795788.1 STAS domain-containing protein -
N288_RS01470 259532..259888 + 357 WP_009795789.1 STAS domain-containing protein -
N288_RS01475 259894..260295 + 402 WP_009795790.1 anti-sigma regulatory factor -
N288_RS01480 260307..261317 + 1011 WP_022543254.1 PP2C family protein-serine/threonine phosphatase -
N288_RS01485 261379..261711 + 333 WP_009795792.1 anti-sigma factor antagonist -
N288_RS01490 261708..262181 + 474 WP_009795793.1 anti-sigma B factor RsbW -
N288_RS01495 262159..262953 + 795 WP_009795794.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13020.06 Da        Isoelectric Point: 4.8998

>T30140 WP_009795787.1 NC_022524:257944-258294 [Bacillus infantis NRRL B-14911]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQISLGLIEF

Download         Length: 351 bp

>T30140 NC_022524:257944-258294 [Bacillus infantis NRRL B-14911]
TTGATTGTCAAACGTGGCGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGTTCAGAACAAGGCGGCGTACGTCCAGT
ACTTGTCATCCAAAACGACATCGGGAATCGGTTCAGTCCCACAGTAATAGTTGCAGCCATTACAGCCCAAATTCAAAAAG
CTAAGCTGCCTACTCACGTTGAAATTGATGCGAAGCGTTACGGTTTTGAACGAGACTCTGTCATTCTGTTGGAACAAATT
CGTACAATTGATAAACAACGCTTAACCGATAAGATAACCCATCTCGATGACGAAATGATGGAAAAAGTGGATGAAGCTTT
GCAAATAAGCTTGGGTCTTATTGAATTCTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10873.41 Da        Isoelectric Point: 7.2029

>AT30140 WP_009795786.1 NC_022524:257658-257939 [Bacillus infantis NRRL B-14911]
VSESSATTEIMVKLPQHLLTELESFVKQENVNRSEFIYQATKMYLRERKKRQIRESMRRGYMEMAKINLTIASEAFQAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT30140 NC_022524:257658-257939 [Bacillus infantis NRRL B-14911]
GTGTCTGAGTCCAGCGCAACAACAGAGATCATGGTAAAGTTACCGCAGCATCTTTTAACCGAGTTAGAAAGCTTTGTTAA
GCAGGAGAATGTCAATCGGAGCGAATTCATTTATCAGGCAACGAAAATGTATTTGCGTGAGCGGAAGAAGAGACAAATTC
GTGAGTCGATGAGAAGAGGCTACATGGAAATGGCAAAAATTAATCTGACGATTGCATCTGAGGCATTCCAAGCGGAATAT
GAGGCAGAACACACAGTTGAACGTCTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U5L4U1

Antitoxin

Source ID Structure
AlphaFold DB A0A2N5FPK8

References