Detailed information of TA system TA030130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2048486..2049000
• Replicon: chromosome
• Accession: NC_022443
• Organism: Staphylococcus aureus subsp. aureus SA40

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: SA40_RS10105
• Protein ID: WP_000581792.1
• Coordinates: 2048486..2048695 (-)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A0D1GBN9
• Locus tag: SA40_RS10110
• Protein ID: WP_000138934.1
• Coordinates: 2048707..2049000 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA40_RS10085	2043994..2045352	-	1359	WP_000611468.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-
SA40_RS10090	2045367..2046437	-	1071	WP_000159641.1	D-alanine--D-alanine ligase	-
SA40_RS10095	2046755..2047957	+	1203	WP_001109939.1	rod shape-determining protein RodA	-
SA40_RS10100	2048221..2048358	-	138	WP_000828354.1	hypothetical protein	-
SA40_RS10105	2048486..2048695	-	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
SA40_RS10110	2048707..2049000	-	294	WP_000138934.1	copper-sensing transcriptional repressor CsoR	Antitoxin
SA40_RS10115	2049166..2050650	+	1485	WP_000571549.1	cardiolipin synthase	-
SA40_RS10120	2050678..2051325	+	648	WP_001187610.1	HD domain-containing protein	-
SA40_RS10130	2052140..2053012	-	873	WP_000725802.1	membrane protein insertase YidC	-
SA40_RS10135	2053098..2053739	-	642	WP_000483147.1	thiamine phosphate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (3-95)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T30130 WP_000581792.1 NC_022443:c2048695-2048486 [Staphylococcus aureus subsp. aureus SA40]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T30130 NC_022443:c2048695-2048486 [Staphylococcus aureus subsp. aureus SA40]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAAACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11185.77 Da
• Isoelectric Point: 6.2243

>AT30130 WP_000138934.1 NC_022443:c2049000-2048707 [Staphylococcus aureus subsp. aureus SA40]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT30130 NC_022443:c2049000-2048707 [Staphylococcus aureus subsp. aureus SA40]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: A0A0D1GBN9