Detailed information of TA system TA030129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2028746..2029275
• Replicon: chromosome
• Accession: NC_022443
• Organism: Staphylococcus aureus subsp. aureus SA40

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SA40_RS10015
• Protein ID: WP_000621175.1
• Coordinates: 2028746..2029108 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SA40_RS10020
• Protein ID: WP_000948331.1
• Coordinates: 2029105..2029275 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA40_RS09990	2025725..2026495	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SA40_RS09995	2026470..2026949	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SA40_RS10000	2026951..2027277	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SA40_RS10005	2027396..2028397	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SA40_RS10015	2028746..2029108	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SA40_RS10020	2029105..2029275	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SA40_RS10025	2029360..2030508	-	1149	WP_001280702.1	alanine racemase	-
SA40_RS10030	2030574..2030933	-	360	WP_000581194.1	holo-ACP synthase	-
SA40_RS10035	2030937..2031428	-	492	WP_001205915.1	PH domain-containing protein	-
SA40_RS10040	2031415..2032998	-	1584	WP_001294642.1	PH domain-containing protein	-
SA40_RS10045	2032991..2033470	-	480	WP_001287083.1	hypothetical protein	-
SA40_RS10050	2033679..2034239	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T30129 WP_000621175.1 NC_022443:c2029108-2028746 [Staphylococcus aureus subsp. aureus SA40]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T30129 NC_022443:c2029108-2028746 [Staphylococcus aureus subsp. aureus SA40]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT30129 WP_000948331.1 NC_022443:c2029275-2029105 [Staphylococcus aureus subsp. aureus SA40]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT30129 NC_022443:c2029275-2029105 [Staphylococcus aureus subsp. aureus SA40]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1