Detailed information of TA system TA030120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2089984..2090513
• Replicon: chromosome
• Accession: NC_022442
• Organism: Staphylococcus aureus subsp. aureus SA957

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SA957_RS10445
• Protein ID: WP_000621175.1
• Coordinates: 2089984..2090346 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SA957_RS10450
• Protein ID: WP_000948331.1
• Coordinates: 2090343..2090513 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SA957_RS10420	2086962..2087732	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SA957_RS10425	2087707..2088186	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SA957_RS10430	2088188..2088514	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SA957_RS10435	2088633..2089634	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SA957_RS10445	2089984..2090346	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SA957_RS10450	2090343..2090513	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SA957_RS10455	2090598..2091746	-	1149	WP_001280702.1	alanine racemase	-
SA957_RS10460	2091812..2092171	-	360	WP_000581194.1	holo-ACP synthase	-
SA957_RS10465	2092175..2092666	-	492	WP_001205915.1	PH domain-containing protein	-
SA957_RS10470	2092653..2094236	-	1584	WP_001294642.1	PH domain-containing protein	-
SA957_RS10475	2094229..2094708	-	480	WP_001287083.1	hypothetical protein	-
SA957_RS10480	2094917..2095477	-	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T30120 WP_000621175.1 NC_022442:c2090346-2089984 [Staphylococcus aureus subsp. aureus SA957]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T30120 NC_022442:c2090346-2089984 [Staphylococcus aureus subsp. aureus SA957]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT30120 WP_000948331.1 NC_022442:c2090513-2090343 [Staphylococcus aureus subsp. aureus SA957]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT30120 NC_022442:c2090513-2090343 [Staphylococcus aureus subsp. aureus SA957]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1