Detailed information of TA system TA030112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 1035824..1036481
• Replicon: chromosome
• Accession: NC_022438
• Organism: Leifsonia xyli subsp. cynodontis DSM 46306

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: O159_RS13845
• Protein ID: WP_081689814.1
• Coordinates: 1036074..1036481 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: O159_RS13840
• Protein ID: WP_081689813.1
• Coordinates: 1035824..1036087 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O159_RS04905	1032141..1033343	+	1203	WP_021754640.1	hypothetical protein	-
O159_RS15195	1033491..1033820	-	330	WP_201766235.1	transposase	-
O159_RS15200	1033780..1034106	-	327	WP_201766236.1	transposase	-
O159_RS14940	1034259..1034519	-	261	WP_021754642.1	IS3 family transposase	-
O159_RS04920	1034527..1034877	-	351	WP_169725660.1	transposase	-
O159_RS14945	1034957..1035118	+	162	WP_169725661.1	hypothetical protein	-
O159_RS13840	1035824..1036087	+	264	WP_081689813.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
O159_RS13845	1036074..1036481	+	408	WP_081689814.1	type II toxin-antitoxin system VapC family toxin	Toxin
O159_RS04925	1036497..1037489	-	993	Protein_988	IS481 family transposase	-
O159_RS14950	1037616..1037792	-	177	WP_169725635.1	hypothetical protein	-
O159_RS04930	1037821..1038186	+	366	WP_021754645.1	DUF4440 domain-containing protein	-
O159_RS04935	1038205..1039317	-	1113	WP_021754646.1	alanine racemase	-
O159_RS04940	1039314..1040024	-	711	WP_043993529.1	alanine racemase	-
O159_RS04945	1040060..1040296	-	237	WP_021754648.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1033491..1037489	3998

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-124)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14697.70 Da
• Isoelectric Point: 5.5934

>T30112 WP_081689814.1 NC_022438:1036074-1036481 [Leifsonia xyli subsp. cynodontis DSM 46306]
MSVTKPIHYFDTSALAKLVVEKGESPALFEWLHAEPRRIVTSDLSRTELLRAVRRVSAYHAATARTLLRACGTVSVSTDI
CDRAALLDPESVRSLDAIHLATALSLGDDLDSFVCYDNRLTEAANLNGLTTVAPE

Nucleotide

• Length: 408 bp

>T30112 NC_022438:1036074-1036481 [Leifsonia xyli subsp. cynodontis DSM 46306]
ATGAGCGTTACTAAGCCCATCCACTATTTCGACACGTCTGCGTTGGCGAAACTCGTTGTGGAAAAAGGTGAGTCGCCGGC
ACTGTTCGAGTGGCTACACGCGGAGCCCCGGAGGATCGTCACATCCGATCTGTCCCGTACCGAACTGCTCCGAGCGGTTC
GTCGGGTCTCAGCGTATCATGCCGCCACAGCACGCACATTGCTGCGTGCGTGCGGAACGGTGTCGGTATCGACCGATATC
TGCGACCGGGCCGCTCTTCTCGACCCGGAGAGCGTTCGCAGCCTGGACGCCATCCATCTTGCAACCGCTCTGTCCCTTGG
CGACGACCTCGACTCATTCGTCTGCTATGACAATCGGCTCACCGAGGCCGCGAACCTGAACGGACTCACGACCGTCGCAC
CCGAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9363.54 Da
• Isoelectric Point: 4.3855

>AT30112 WP_081689813.1 NC_022438:1035824-1036087 [Leifsonia xyli subsp. cynodontis DSM 46306]
MYMVGIRELKQNPSAVVADAAAGETVIISDRGRPVARLTPLPQSSWAGLVESGLAHDPVLPIASITYPTSEPSVSKALEE
DRDDERY

Nucleotide

• Length: 264 bp

>AT30112 NC_022438:1035824-1036087 [Leifsonia xyli subsp. cynodontis DSM 46306]
ATGTACATGGTGGGGATCCGAGAGTTGAAACAGAACCCTTCAGCTGTTGTCGCGGACGCGGCGGCTGGTGAAACGGTCAT
TATCTCGGATCGAGGTCGGCCCGTTGCGCGTTTGACTCCTTTGCCTCAGTCGTCGTGGGCTGGGTTAGTGGAATCCGGGC
TCGCCCACGATCCGGTCTTGCCGATCGCCTCGATCACTTACCCGACCTCGGAGCCGAGTGTGTCGAAAGCTCTGGAAGAG
GACCGCGACGATGAGCGTTACTAA