Detailed information of TA system TA030110

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 4708752..4709459
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: E0J2C8
• Locus tag: LY180_RS23265
• Protein ID: WP_000691790.1
• Coordinates: 4709118..4709459 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LY180_RS23260
• Protein ID: WP_000939438.1
• Coordinates: 4708752..4709087 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS23235	4704699..4704917	-	219	WP_001064742.1	AlpA family phage regulatory protein	-
LY180_RS23240	4705035..4705649	-	615	WP_000772909.1	inovirus Gp2 family protein	-
LY180_RS23245	4706242..4707195	+	954	WP_000290405.1	hypothetical protein	-
LY180_RS23250	4707431..4708249	+	819	WP_000761991.1	DUF945 domain-containing protein	-
LY180_RS23255	4708279..4708752	+	474	WP_001292742.1	DNA repair protein RadC	-
LY180_RS23260	4708752..4709087	+	336	WP_000939438.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LY180_RS23265	4709118..4709459	+	342	WP_000691790.1	TA system toxin CbtA family protein	Toxin
LY180_RS23270	4709574..4710407	+	834	WP_001192746.1	DUF4942 domain-containing protein	-
LY180_RS23275	4710477..4710872	+	396	WP_000152741.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
LY180_RS23280	4710865..4711812	+	948	WP_000342500.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
LY180_RS23285	4712205..4713470	+	1266	WP_001218329.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB / fimE / fimA / fimI / fimC	4693160..4742153	48993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (10-110)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13060.08 Da
• Isoelectric Point: 9.4583

>T30110 WP_000691790.1 NC_022364:4709118-4709459 [Escherichia coli LY180]
MKIIPATTLRATTSYLSPVVVWQTLLARLLEQHYGLNLNDTPFNNEKVIQEHIDAGITLVDAVNFLVEKYELIRIDRKGF
SWQEQTPYLRAVDILRARQATGLLRRCYNTTAR

Nucleotide

• Length: 342 bp

>T30110 NC_022364:4709118-4709459 [Escherichia coli LY180]
ATGAAAATTATACCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCGCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCGATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGATCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCTACAGGTCTGCTGCGCCGCTG
CTATAACACCACGGCACGGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12639.63 Da
• Isoelectric Point: 7.7723

>AT30110 WP_000939438.1 NC_022364:4708752-4709087 [Escherichia coli LY180]
MLQKDKVMQQEWGLPCTVTPRFTARLVQERHNLHYLADRAAITGKFSSPDLLHIDQAFPVLMKQLELKLADGELSPWQQR
TVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 336 bp

>AT30110 NC_022364:4708752-4709087 [Escherichia coli LY180]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGCTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGGCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA

Structures

Toxin

• AlphaFold DB: E0J2C8