Detailed information of TA system TA030105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3709236..3710036
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: LY180_RS18400
• Protein ID: WP_000342449.1
• Coordinates: 3709236..3709763 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: LY180_RS18405
• Protein ID: WP_001277108.1
• Coordinates: 3709770..3710036 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS18375	3704311..3705078	-	768	WP_000082110.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LY180_RS18380	3705075..3706352	-	1278	WP_000803798.1	branched chain amino acid ABC transporter permease LivM	-
LY180_RS18385	3706349..3707275	-	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LY180_RS18390	3707323..3708432	-	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LY180_RS18395	3708856..3709239	+	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LY180_RS18400	3709236..3709763	-	528	WP_000342449.1	GNAT family N-acetyltransferase	Toxin
LY180_RS18405	3709770..3710036	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
LY180_RS18410	3710186..3711289	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LY180_RS18415	3711560..3712414	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LY180_RS18420	3712659..3713717	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LY180_RS18425	3713710..3714378	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T30105 WP_000342449.1 NC_022364:c3709763-3709236 [Escherichia coli LY180]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T30105 NC_022364:c3709763-3709236 [Escherichia coli LY180]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATAAAAAAGCCCATACGTTTTATCAATCGCTGGGCTTTATCCCGTTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATCGAACTGCTTTTCACACAGAGCGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT30105 WP_001277108.1 NC_022364:c3710036-3709770 [Escherichia coli LY180]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT30105 NC_022364:c3710036-3709770 [Escherichia coli LY180]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCTGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24