Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relE-higA/Xre-RelE |
| Location | 333189..334363 | Replicon | chromosome |
| Accession | NC_002947 | ||
| Organism | Pseudomonas putida KT2440 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | Q88R59 |
| Locus tag | PP_5435 | Protein ID | 26987017 |
| Coordinates | 332862..333215 (+) | Length | 118 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | PP_0274 | Protein ID | 26987016 |
| Coordinates | 333257..333730 (+) | Length | 158 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| PP_RS01425 (PP_0271) | 329251..329970 | - | 720 | WP_010951640.1 | response regulator | - |
| PP_RS01430 (PP_0272) | 330111..332408 | + | 2298 | WP_010951641.1 | TonB-dependent siderophore receptor | - |
| PP_RS01435 (PP_0273) | 332474..332680 | - | 207 | WP_014860378.1 | DUF3079 domain-containing protein | - |
| PP_RS01440 (PP_5435) | 332862..333215 | + | 354 | WP_061405554.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| PP_RS01445 (PP_0274) | 333257..333730 | + | 474 | WP_004575922.1 | transcriptional regulator | Antitoxin |
| PP_RS01450 (PP_5436) | 333735..333922 | - | 188 | Protein_280 | integrase | - |
| PP_RS01455 (PP_0275) | 334150..334365 | + | 216 | WP_010951644.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| PP_RS01460 (PP_0276) | 334362..334685 | - | 324 | WP_010951645.1 | helix-turn-helix domain-containing protein | - |
| PP_RS01470 (PP_0277) | 335147..335395 | - | 249 | WP_010951646.1 | hypothetical protein | - |
| PP_RS01475 (PP_0278) | 335916..336190 | + | 275 | Protein_284 | DUF3077 domain-containing protein | - |
| PP_RS01480 (PP_0279) | 336545..337750 | - | 1206 | WP_010951648.1 | methyltransferase | - |
| PP_RS01485 (PP_0280) | 337879..338568 | - | 690 | WP_010951649.1 | ABC transporter permease | - |
| PP_RS01490 (PP_0281) | 338568..339260 | - | 693 | WP_010951650.1 | ABC transporter permease | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 118 a.a. Molecular weight: 13672.88 Da Isoelectric Point: 10.6182
>T301 26987017 NC_002947:332862-333215 [Pseudomonas putida KT2440]
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR
MRVITKAAVTKAIEVHGQWKAPLSLWLTTFDRATLRFESFEQLRQTWATVSGWNVDRIPHSKLRPASRKGPLDIYVFDIK
KNECRLIAWLNARTGTLYIKAILSHAEYDKWCRSDIR
Download Length: 354 bp
>T301 NC_002947:332862-333215 [Pseudomonas putida KT2440]
ATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGATTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCTTTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG
ATGCGGGTCATAACGAAAGCGGCGGTTACCAAGGCGATTGAAGTCCACGGCCAGTGGAAAGCGCCCCTAAGCCTGTGGCT
GACGACCTTCGACAGGGCGACGCTGCGGTTCGAATCTTTTGAACAGTTGAGGCAGACGTGGGCCACTGTGTCTGGCTGGA
ATGTTGATCGCATCCCCCACTCAAAACTCAGGCCCGCCAGCCGCAAAGGGCCGCTTGATATCTACGTGTTCGATATCAAG
AAAAACGAATGCCGCCTGATCGCGTGGCTCAATGCGCGAACGGGTACTCTCTACATCAAGGCCATTTTGTCGCACGCCGA
GTACGACAAATGGTGCAGAAGCGATATCAGGTAG
Antitoxin
Download Length: 158 a.a. Molecular weight: 17229.67 Da Isoelectric Point: 4.4982
>AT301 26987016 NC_002947:333257-333730 [Pseudomonas putida KT2440]
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV
MKRDKIEANGLDAFIANISPMLDGLNTQMATMAQLLAACHDPIKDDAELEARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLSAIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV
Download Length: 474 bp
>AT301 NC_002947:333257-333730 [Pseudomonas putida KT2440]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTCGATGGCCTGAACAC
TCAGATGGCAACCATGGCCCAGCTTCTAGCCGCCTGCCATGACCCCATCAAGGACGATGCCGAGCTTGAGGCACGCATGG
CCTTGATCGACGAGCTCTACTCCCATGCGGACAGCGAAGGCCACGCTGCGGCACGCTTTGCCGAGATGGTAGCGGACCGC
GTCTACGAGTATGAGAGTGAGTCTGTATTGGTGCCGTTCGCGAGCCAGGCCGAAGCGTTGGCCTTTCTGCTGTCGGATAG
GGGCGTGAAGCAGAAGGACCTGTCGGCGATAGCGACCCAGAGCGCGGTATCCGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTACCGGTTGAGTTCTTCATGCATGGGGTGGTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
References
(1) Sirli Rosendahl et al. (2020) Chromosomal toxin-antitoxin systems in Pseudomonas putida are rather selfish than beneficial. Scientific Reports 10(1):9230. [PubMed:32513960]