Detailed information of TA system TA030091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 1718932..1719458
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: LY180_RS08435
• Protein ID: WP_000323025.1
• Coordinates: 1718932..1719219 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: LY180_RS08440
• Protein ID: WP_000534858.1
• Coordinates: 1719219..1719458 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS08395	1713956..1714171	-	216	WP_000839590.1	phage lysis protein EssD	-
LY180_RS08400	1714925..1715140	-	216	WP_000066484.1	cold shock-like protein CspB	-
LY180_RS08405	1715441..1715653	+	213	WP_000087756.1	cold shock-like protein CspF	-
LY180_RS26300	1715708..1715797	+	90	WP_120795389.1	hypothetical protein	-
LY180_RS08410	1716075..1716827	-	753	WP_001047135.1	antitermination protein	-
LY180_RS08415	1716841..1717890	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
LY180_RS08420	1717892..1718170	-	279	WP_012304870.1	hypothetical protein	-
LY180_RS08425	1718237..1718488	-	252	WP_000980994.1	hypothetical protein	-
LY180_RS08430	1718705..1718860	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
LY180_RS08435	1718932..1719219	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
LY180_RS08440	1719219..1719458	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
LY180_RS24640	1719483..1719788	+	306	WP_001326990.1	hypothetical protein	-
LY180_RS08445	1719991..1720323	+	333	WP_001301033.1	protein FlxA	-
LY180_RS08450	1720760..1722073	-	1314	WP_000589005.1	ISNCY family transposase	-
LY180_RS26710	1722842..1723129	-	288	Protein_1623	hypothetical protein	-
LY180_RS08465	1723740..1724096	-	357	WP_001310834.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1675294..1733959	58665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T30091 WP_000323025.1 NC_022364:c1719219-1718932 [Escherichia coli LY180]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T30091 NC_022364:c1719219-1718932 [Escherichia coli LY180]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT30091 WP_000534858.1 NC_022364:c1719458-1719219 [Escherichia coli LY180]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT30091 NC_022364:c1719458-1719219 [Escherichia coli LY180]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8