Detailed information of TA system TA030089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1568349..1568987
• Replicon: chromosome
• Accession: NC_022364
• Organism: Escherichia coli LY180

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: LY180_RS07720
• Protein ID: WP_000813794.1
• Coordinates: 1568349..1568525 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LY180_RS07725
• Protein ID: WP_001270285.1
• Coordinates: 1568571..1568987 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY180_RS07700	1563968..1565182	-	1215	WP_001326689.1	BenE family transporter YdcO	-
LY180_RS07705	1565235..1565771	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
LY180_RS07710	1565844..1567805	+	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LY180_RS07715	1567897..1568127	-	231	WP_000494244.1	YncJ family protein	-
LY180_RS07720	1568349..1568525	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LY180_RS07725	1568571..1568987	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LY180_RS07730	1569066..1570472	+	1407	WP_000760593.1	PLP-dependent aminotransferase family protein	-
LY180_RS07735	1570717..1571862	+	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
LY180_RS07740	1571880..1572893	+	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
LY180_RS07745	1572894..1573835	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1562780..1563928	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T30089 WP_000813794.1 NC_022364:1568349-1568525 [Escherichia coli LY180]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T30089 NC_022364:1568349-1568525 [Escherichia coli LY180]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT30089 WP_001270285.1 NC_022364:1568571-1568987 [Escherichia coli LY180]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT30089 NC_022364:1568571-1568987 [Escherichia coli LY180]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB