Detailed information of TA system TA030081

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 2582273..2582784
• Replicon: chromosome
• Accession: NC_022357
• Organism: Sulfuricella denitrificans skB26

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S6ADB3
• Locus tag: SCD_RS12490
• Protein ID: WP_009207713.1
• Coordinates: 2582273..2582527 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S6ANB2
• Locus tag: SCD_RS12495
• Protein ID: WP_009207712.1
• Coordinates: 2582524..2582784 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCD_RS12460	2577714..2578742	-	1029	WP_009207719.1	N-acetyl-gamma-glutamyl-phosphate reductase	-
SCD_RS12465	2578865..2579257	-	393	WP_009207718.1	30S ribosomal protein S9	-
SCD_RS12470	2579267..2579695	-	429	WP_009207717.1	50S ribosomal protein L13	-
SCD_RS12475	2579827..2580243	+	417	WP_009207716.1	OsmC family protein	-
SCD_RS12480	2580375..2581001	-	627	WP_009207715.1	2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase	-
SCD_RS12485	2580988..2582241	-	1254	WP_023507005.1	(Fe-S)-binding protein	-
SCD_RS12490	2582273..2582527	-	255	WP_009207713.1	Txe/YoeB family addiction module toxin	Toxin
SCD_RS12495	2582524..2582784	-	261	WP_009207712.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
SCD_RS12500	2582921..2584069	-	1149	WP_009207711.1	porin	-
SCD_RS12505	2584381..2585328	-	948	WP_009207710.1	indole-3-glycerol phosphate synthase TrpC	-
SCD_RS12510	2585413..2586441	-	1029	WP_009207709.1	anthranilate phosphoribosyltransferase	-
SCD_RS12515	2586536..2586916	-	381	WP_023507006.1	endonuclease domain-containing protein	-
SCD_RS12520	2587105..2587674	-	570	WP_009207707.1	aminodeoxychorismate/anthranilate synthase component II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-70)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10165.70 Da
• Isoelectric Point: 8.4985

>T30081 WP_009207713.1 NC_022357:c2582527-2582273 [Sulfuricella denitrificans skB26]
MKILFHPHAWEDYGYWLQADPRKLKRINALIRDIQRSPFEGIGKPEGLRFDLSGCWSRRIDDEHRLVYRIEADVLHLLAC
RYHY

Nucleotide

• Length: 255 bp

>T30081 NC_022357:c2582527-2582273 [Sulfuricella denitrificans skB26]
ATGAAAATCCTGTTTCACCCACATGCCTGGGAAGATTACGGCTACTGGTTGCAAGCCGATCCCAGGAAGTTAAAGCGCAT
CAATGCGCTGATACGAGATATTCAACGCTCTCCCTTTGAAGGTATCGGTAAGCCGGAAGGGCTGCGCTTCGATCTTTCGG
GTTGCTGGAGTCGTCGGATTGACGACGAGCATAGGCTGGTTTATCGGATCGAAGCGGATGTGCTGCATCTGCTTGCCTGT
CGTTATCATTATTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9748.05 Da
• Isoelectric Point: 5.1693

>AT30081 WP_009207712.1 NC_022357:c2582784-2582524 [Sulfuricella denitrificans skB26]
MEALSYSELRSNLKKVMDRVCDTHEPVIVVRRNGEKTVMLSYADYSAIEETSYLMRSPKMAARLRESLESLHNGGGEERP
LTDVGE

Nucleotide

• Length: 261 bp

>AT30081 NC_022357:c2582784-2582524 [Sulfuricella denitrificans skB26]
ATGGAAGCGCTTTCTTATAGCGAATTGCGGAGTAATTTGAAGAAGGTGATGGACAGGGTGTGCGATACGCACGAACCGGT
CATTGTCGTTCGGCGCAATGGAGAGAAGACGGTAATGCTTTCGTACGCTGATTATTCGGCTATCGAAGAAACGTCTTACC
TGATGAGGAGTCCGAAAATGGCTGCACGTCTGCGTGAAAGTCTGGAAAGCTTGCACAATGGAGGCGGTGAAGAGAGGCCG
TTGACTGACGTGGGCGAATGA

Structures

Toxin

• AlphaFold DB: S6ADB3

Antitoxin

• AlphaFold DB: S6ANB2