Detailed information of TA system TA030076

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 77960..78618
• Replicon: chromosome
• Accession: NC_022357
• Organism: Sulfuricella denitrificans skB26

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: S6AHE9
• Locus tag: SCD_RS00330
• Protein ID: WP_009207141.1
• Coordinates: 77960..78373 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: S6A9D4
• Locus tag: SCD_RS00335
• Protein ID: WP_009207140.1
• Coordinates: 78370..78618 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SCD_RS00320	74067..76430	+	2364	WP_009207143.1	DUF1631 domain-containing protein	-
SCD_RS00325	76526..77869	+	1344	WP_041673260.1	EAL domain-containing protein	-
SCD_RS00330	77960..78373	-	414	WP_009207141.1	type II toxin-antitoxin system VapC family toxin	Toxin
SCD_RS00335	78370..78618	-	249	WP_009207140.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SCD_RS00340	78662..79681	-	1020	WP_009207139.1	hypothetical protein	-
SCD_RS00345	79678..79977	-	300	WP_009207138.1	YggU family protein	-
SCD_RS00350	79981..80556	-	576	WP_009207137.1	YggT family protein	-
SCD_RS00355	80559..81388	-	830	Protein_69	pyrroline-5-carboxylate reductase	-
SCD_RS00360	81502..82191	-	690	WP_041673261.1	YggS family pyridoxal phosphate-dependent enzyme	-
SCD_RS00365	82228..83271	+	1044	WP_009207133.1	type IV pilus twitching motility protein PilT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-62)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15701.03 Da
• Isoelectric Point: 5.6789

>T30076 WP_009207141.1 NC_022357:c78373-77960 [Sulfuricella denitrificans skB26]
MSYLIDTNVIFELARAKPDPRVVEWFNTIPDSALYLSVLTLGELRKRVEKMPDANRREKLRLWLENDLPAWFGDHLLPVN
AAIADRWGRLQSETTRPLPSADSLLTATALHHDLRIVTCNDKDFEVPGLVVVNPWKD

Nucleotide

• Length: 414 bp

>T30076 NC_022357:c78373-77960 [Sulfuricella denitrificans skB26]
ATGAGCTACCTCATCGACACCAACGTCATTTTCGAACTCGCCCGAGCCAAGCCCGATCCACGAGTGGTGGAATGGTTTAA
TACCATACCGGATTCTGCCCTGTACTTGAGCGTCCTGACCTTGGGGGAATTGCGTAAAAGAGTGGAGAAGATGCCGGATG
CAAACCGCCGGGAAAAACTGCGTCTGTGGCTGGAAAACGACTTGCCTGCCTGGTTCGGCGATCATCTATTACCTGTAAAC
GCCGCCATCGCCGACCGGTGGGGCCGCCTGCAGTCCGAAACCACTCGCCCCCTTCCCTCTGCCGATAGCCTGCTGACGGC
CACCGCCCTGCATCACGACTTGCGGATAGTCACATGCAACGATAAGGATTTTGAGGTCCCGGGTTTGGTGGTGGTAAATC
CGTGGAAGGATTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9300.60 Da
• Isoelectric Point: 5.6805

>AT30076 WP_009207140.1 NC_022357:c78618-78370 [Sulfuricella denitrificans skB26]
MQAWQLQEAKARLSEVIQKAINEGPQDISLRGRPAAMILSHADYEMLTRPKPSFTAFLRDSPLHGVELEWEPDTALTREV
GR

Nucleotide

• Length: 249 bp

>AT30076 NC_022357:c78618-78370 [Sulfuricella denitrificans skB26]
ATGCAGGCATGGCAACTGCAAGAAGCGAAAGCCCGCTTGAGCGAGGTAATTCAGAAAGCGATTAACGAAGGGCCGCAGGA
CATCTCCCTGCGGGGGCGCCCGGCGGCGATGATCCTTTCTCATGCCGATTATGAGATGCTAACCCGACCCAAGCCTTCCT
TCACCGCCTTTCTGCGCGACTCCCCCCTGCACGGCGTTGAACTCGAATGGGAGCCGGACACCGCCCTCACCCGCGAGGTT
GGACGATGA

Structures

Toxin

• AlphaFold DB: S6AHE9

Antitoxin

• AlphaFold DB: S6A9D4