Detailed information of TA system TA030071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): toxTA/Tad-couple_hipB
• Location: 295904..296552
• Replicon: chromosome
• Accession: NC_022356
• Organism: Haemophilus influenzae KR494

Toxin (Protein)

• Gene name: toxT
• Uniprot ID: A0A0Y7JUP0
• Locus tag: HIFGL_RS01475
• Protein ID: WP_005694481.1
• Coordinates: 296193..296552 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: HIFGL_RS01470
• Protein ID: WP_005650837.1
• Coordinates: 295904..296200 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIFGL_RS01445	291131..291694	-	564	WP_021034570.1	DNA-3-methyladenine glycosylase I	-
HIFGL_RS01450	291908..292714	-	807	WP_021034572.1	shikimate dehydrogenase	-
HIFGL_RS01455	292718..293269	-	552	WP_021034573.1	Sua5/YciO/YrdC/YwlC family protein	-
HIFGL_RS01460	293285..293821	-	537	WP_021034574.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
HIFGL_RS01465	293831..295747	-	1917	WP_021034575.1	ABC transporter ATP-binding protein	-
HIFGL_RS01470	295904..296200	-	297	WP_005650837.1	helix-turn-helix transcriptional regulator	Antitoxin
HIFGL_RS01475	296193..296552	-	360	WP_005694481.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIFGL_RS01480	296813..299788	-	2976	WP_021034576.1	TonB-dependent hemoglobin/transferrin/lactoferrin family receptor	-
HIFGL_RS01485	300151..300315	+	165	WP_021034577.1	hypothetical protein	-
HIFGL_RS01490	300364..300528	+	165	WP_021034578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (36-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (34-89)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 14321.67 Da
• Isoelectric Point: 10.1408

>T30071 WP_005694481.1 NC_022356:c296552-296193 [Haemophilus influenzae KR494]
MYEILFYRDQNDIEPVKEYLLSLAQNESKDSRIKLNKIRDYVKLLSELGTSVGKPYVKHLDGEIWELRPIRDRILFARLM
DGRFVLLHQFMKKTQKTPKREIQTAQQRLSELKERLKNE

Nucleotide

• Length: 360 bp

>T30071 NC_022356:c296552-296193 [Haemophilus influenzae KR494]
ATGTACGAGATTCTCTTTTACAGAGACCAAAATGACATTGAGCCAGTGAAAGAGTATCTGCTATCTCTGGCTCAAAATGA
AAGCAAAGATAGTCGAATTAAACTGAATAAAATTCGAGATTATGTAAAGTTATTAAGCGAACTCGGGACGTCTGTTGGCA
AACCCTATGTGAAACATTTAGACGGTGAAATTTGGGAACTTCGTCCAATTAGGGATCGCATCTTGTTTGCAAGATTAATG
GATGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAACGAGAAATCCAAACAGCCCAACA
ACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11092.94 Da
• Isoelectric Point: 10.3139

>AT30071 WP_005650837.1 NC_022356:c296200-295904 [Haemophilus influenzae KR494]
MNKISPLGSNWNEFEQQIFNEEEIRESNLRVALIKELITSRQQLGISQKQLETLSGVKQPMIARIEKGQTNPQLETLLKL
LAPLGKTLSIVPLRVKNA

Nucleotide

• Length: 297 bp

>AT30071 NC_022356:c296200-295904 [Haemophilus influenzae KR494]
ATGAATAAAATTAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGCCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAGCTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A0Y7JUP0