Detailed information of TA system TA030068

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3765816..3766345
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: TBHG_RS17630
• Protein ID: WP_003417760.1
• Coordinates: 3766088..3766345 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: TBHG_RS17625
• Protein ID: WP_003417757.1
• Coordinates: 3765816..3766091 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS22510	3762614..3764051	-	1438	Protein_3453	FAD-binding oxidoreductase	-
TBHG_RS17610	3764154..3764543	+	390	WP_003417745.1	DUF732 domain-containing protein	-
TBHG_RS17615	3764557..3764850	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
TBHG_RS17620	3764847..3765692	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
TBHG_RS17625	3765816..3766091	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
TBHG_RS17630	3766088..3766345	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
TBHG_RS17635	3766387..3767577	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
TBHG_RS17640	3767694..3768062	+	369	WP_003417765.1	FHA domain-containing protein	-
TBHG_RS17645	3768059..3768610	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
TBHG_RS17650	3768617..3769198	-	582	WP_003417769.1	ATP/GTP-binding protein	-
TBHG_RS17655	3769179..3769547	-	369	WP_003417772.1	DUF742 domain-containing protein	-
TBHG_RS17660	3769525..3769917	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T30068 WP_003417760.1 NC_022350:3766088-3766345 [Mycobacterium tuberculosis str. Haarlem]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T30068 NC_022350:3766088-3766345 [Mycobacterium tuberculosis str. Haarlem]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT30068 WP_003417757.1 NC_022350:3765816-3766091 [Mycobacterium tuberculosis str. Haarlem]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT30068 NC_022350:3765816-3766091 [Mycobacterium tuberculosis str. Haarlem]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94