Detailed information of TA system TA030061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3104227..3104797
• Replicon: chromosome
• Accession: NC_022350
• Organism: Mycobacterium tuberculosis str. Haarlem

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: TBHG_RS14670
• Protein ID: WP_003414166.1
• Coordinates: 3104227..3104583 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: TBHG_RS14675
• Protein ID: WP_003901465.1
• Coordinates: 3104567..3104797 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBHG_RS14650	3099679..3101367	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
TBHG_RS14655	3101371..3101697	-	327	WP_003414157.1	hypothetical protein	-
TBHG_RS14660	3101870..3102457	+	588	WP_003914429.1	DUF3558 family protein	-
TBHG_RS14665	3102476..3104125	+	1650	WP_003900576.1	CocE/NonD family hydrolase	-
TBHG_RS14670	3104227..3104583	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
TBHG_RS14675	3104567..3104797	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
TBHG_RS14680	3104840..3105883	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
TBHG_RS22120	3106074..3106349	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
TBHG_RS22465	3106525..3106779	-	255	WP_003917684.1	hypothetical protein	-
TBHG_RS14695	3106927..3107331	+	405	WP_009938577.1	hypothetical protein	-
TBHG_RS14700	3107328..3107519	+	192	WP_003414184.1	hypothetical protein	-
TBHG_RS14705	3107583..3108872	+	1290	Protein_2887	transposase family protein	-
TBHG_RS14710	3109106..3109363	+	258	WP_003899489.1	hypothetical protein	-
TBHG_RS14715	3109468..3109779	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3094262..3117245	22983

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T30061 WP_003414166.1 NC_022350:c3104583-3104227 [Mycobacterium tuberculosis str. Haarlem]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T30061 NC_022350:c3104583-3104227 [Mycobacterium tuberculosis str. Haarlem]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT30061 WP_003901465.1 NC_022350:c3104797-3104567 [Mycobacterium tuberculosis str. Haarlem]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT30061 NC_022350:c3104797-3104567 [Mycobacterium tuberculosis str. Haarlem]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3